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Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form

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ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.


Related in: MedlinePlus

Embryo attachment and ENPP3 expression.Human embryo attached to the in vitro three-dimensional cell culture model with progesterone exposure (A). None of the 8 embryos in the anti-progestin treated group, where ENPP3 was significantly down regulated had attached. In the control group, 7 out of 10 blastocysts attached (B) to the construct, with very good expression of ENPP3 in the three-dimensional endometrial cell culture system.
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f7: Embryo attachment and ENPP3 expression.Human embryo attached to the in vitro three-dimensional cell culture model with progesterone exposure (A). None of the 8 embryos in the anti-progestin treated group, where ENPP3 was significantly down regulated had attached. In the control group, 7 out of 10 blastocysts attached (B) to the construct, with very good expression of ENPP3 in the three-dimensional endometrial cell culture system.

Mentions: Embryo attachment was studied in a previously-described three-dimensional endometrial cell culture model to study human implantation process in vitro32, and showed that 7 out of 10 blastocysts attached to the endometrial construct in the control group. In controls, a very good expression level of ENPP3 was seen as studied by real-time PCR. Exposure to mifepristone (0.5 μM) led to significant (p = 0.004; fisher’s exact test) down-regulation of ENPP3, with none of the embryos attached to the in vitro endometrial construct (p = 0.004, Fig. 7).


Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form
Embryo attachment and ENPP3 expression.Human embryo attached to the in vitro three-dimensional cell culture model with progesterone exposure (A). None of the 8 embryos in the anti-progestin treated group, where ENPP3 was significantly down regulated had attached. In the control group, 7 out of 10 blastocysts attached (B) to the construct, with very good expression of ENPP3 in the three-dimensional endometrial cell culture system.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036034&req=5

f7: Embryo attachment and ENPP3 expression.Human embryo attached to the in vitro three-dimensional cell culture model with progesterone exposure (A). None of the 8 embryos in the anti-progestin treated group, where ENPP3 was significantly down regulated had attached. In the control group, 7 out of 10 blastocysts attached (B) to the construct, with very good expression of ENPP3 in the three-dimensional endometrial cell culture system.
Mentions: Embryo attachment was studied in a previously-described three-dimensional endometrial cell culture model to study human implantation process in vitro32, and showed that 7 out of 10 blastocysts attached to the endometrial construct in the control group. In controls, a very good expression level of ENPP3 was seen as studied by real-time PCR. Exposure to mifepristone (0.5 μM) led to significant (p = 0.004; fisher’s exact test) down-regulation of ENPP3, with none of the embryos attached to the in vitro endometrial construct (p = 0.004, Fig. 7).

View Article: PubMed Central - PubMed

ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.


Related in: MedlinePlus