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Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form

View Article: PubMed Central - PubMed

ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.


Heatmaps for hierarchical clustering of significant genes.Genes regulated by progesterone in endometrial glandular (G, left panel) and stromal (S, right panel) compartments shown by heatmaps. Gene expression was studied in the receptive endometrium (C) in a non-treated cycle and non-receptive endometrium (T) with the treatment of progesterone receptor antagonist mifepristone. Each woman in the study acted as their own control.
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f1: Heatmaps for hierarchical clustering of significant genes.Genes regulated by progesterone in endometrial glandular (G, left panel) and stromal (S, right panel) compartments shown by heatmaps. Gene expression was studied in the receptive endometrium (C) in a non-treated cycle and non-receptive endometrium (T) with the treatment of progesterone receptor antagonist mifepristone. Each woman in the study acted as their own control.

Mentions: The LCMD of approximately 200 cells yielded a minimum of 500 pg of RNA that was taken for amplification. Microarray data analysis showed 32 genes out of 47 were up-regulated and 15 genes down-regulated in the epithelial compartment, whereas in the stromal compartment, 79 out of 85 genes were up-regulated and 6 down-regulated with mifepristone treatment (Supplementary Table 1). A higher number of significantly downregulated genes were observed in the glands in comparison to stroma with mifepristone treatment (Fig. 1).


Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form
Heatmaps for hierarchical clustering of significant genes.Genes regulated by progesterone in endometrial glandular (G, left panel) and stromal (S, right panel) compartments shown by heatmaps. Gene expression was studied in the receptive endometrium (C) in a non-treated cycle and non-receptive endometrium (T) with the treatment of progesterone receptor antagonist mifepristone. Each woman in the study acted as their own control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036034&req=5

f1: Heatmaps for hierarchical clustering of significant genes.Genes regulated by progesterone in endometrial glandular (G, left panel) and stromal (S, right panel) compartments shown by heatmaps. Gene expression was studied in the receptive endometrium (C) in a non-treated cycle and non-receptive endometrium (T) with the treatment of progesterone receptor antagonist mifepristone. Each woman in the study acted as their own control.
Mentions: The LCMD of approximately 200 cells yielded a minimum of 500 pg of RNA that was taken for amplification. Microarray data analysis showed 32 genes out of 47 were up-regulated and 15 genes down-regulated in the epithelial compartment, whereas in the stromal compartment, 79 out of 85 genes were up-regulated and 6 down-regulated with mifepristone treatment (Supplementary Table 1). A higher number of significantly downregulated genes were observed in the glands in comparison to stroma with mifepristone treatment (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.