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Sporicidal performance induced by photocatalytic production of organic peroxide under visible light irradiation

View Article: PubMed Central - PubMed

ABSTRACT

Bacteria that cause serious food poisoning are known to sporulate under conditions of nutrient and water shortage. The resulting spores have much greater resistance to common sterilization methods, such as heating at 100 °C and exposure to various chemical agents. Because such bacteria cannot be inactivated with typical alcohol disinfectants, peroxyacetic acid (PAA) often is used, but PAA is a harmful agent that can seriously damage human health. Furthermore, concentrated hydrogen peroxide, which is also dangerous, must be used to prepare PAA. Thus, the development of a facile and safe sporicidal disinfectant is strongly required. In this study, we have developed an innovative sporicidal disinfection method that employs the combination of an aqueous ethanol solution, visible light irradiation, and a photocatalyst. We successfully produced a sporicidal disinfectant one hundred times as effective as commercially available PAA, while also resolving the hazards and odor problems associated with PAA. The method presented here can potentially be used as a replacement for the general disinfectants employed in the food and health industries.

No MeSH data available.


Time-dependence of amount of organic peroxide, hydrogen peroxide, acetic acid, and formic acid produced by WO3 suspended in ethanol:water solution (8:2, v/v) illuminated with visible light (λ > 420 nm) for the indicated time.
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f3: Time-dependence of amount of organic peroxide, hydrogen peroxide, acetic acid, and formic acid produced by WO3 suspended in ethanol:water solution (8:2, v/v) illuminated with visible light (λ > 420 nm) for the indicated time.

Mentions: Therefore, the level of PAA can be quantified measuring the amount of MTSO generated. This method has the great advantage because the detection of PAA is not inhibited with the coexistence of hydrogen peroxide which typically oxidizes organic chemicals. A portion of the WO3 suspension after photocatalytic reaction was added to an MTS solution and the presence of MTSO was detected as shown in Fig. 2. The amount of organic peroxide produced gradually increased during the photocatalytic reaction, as shown in Fig. 3. The amount of organic peroxide produced after 12 h of visible light irradiation was estimated to be ca. 8.84 μmol (ca. 13.5 ppm), confirming the successful production of organic peroxide by photocatalytic oxidation of aqueous ethanol solution by WO3 illuminated with visible light. The organic peroxide was generated by an equilibrium reaction between hydrogen peroxide and organic acids [Equation 2]263940.


Sporicidal performance induced by photocatalytic production of organic peroxide under visible light irradiation
Time-dependence of amount of organic peroxide, hydrogen peroxide, acetic acid, and formic acid produced by WO3 suspended in ethanol:water solution (8:2, v/v) illuminated with visible light (λ > 420 nm) for the indicated time.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036025&req=5

f3: Time-dependence of amount of organic peroxide, hydrogen peroxide, acetic acid, and formic acid produced by WO3 suspended in ethanol:water solution (8:2, v/v) illuminated with visible light (λ > 420 nm) for the indicated time.
Mentions: Therefore, the level of PAA can be quantified measuring the amount of MTSO generated. This method has the great advantage because the detection of PAA is not inhibited with the coexistence of hydrogen peroxide which typically oxidizes organic chemicals. A portion of the WO3 suspension after photocatalytic reaction was added to an MTS solution and the presence of MTSO was detected as shown in Fig. 2. The amount of organic peroxide produced gradually increased during the photocatalytic reaction, as shown in Fig. 3. The amount of organic peroxide produced after 12 h of visible light irradiation was estimated to be ca. 8.84 μmol (ca. 13.5 ppm), confirming the successful production of organic peroxide by photocatalytic oxidation of aqueous ethanol solution by WO3 illuminated with visible light. The organic peroxide was generated by an equilibrium reaction between hydrogen peroxide and organic acids [Equation 2]263940.

View Article: PubMed Central - PubMed

ABSTRACT

Bacteria that cause serious food poisoning are known to sporulate under conditions of nutrient and water shortage. The resulting spores have much greater resistance to common sterilization methods, such as heating at 100 °C and exposure to various chemical agents. Because such bacteria cannot be inactivated with typical alcohol disinfectants, peroxyacetic acid (PAA) often is used, but PAA is a harmful agent that can seriously damage human health. Furthermore, concentrated hydrogen peroxide, which is also dangerous, must be used to prepare PAA. Thus, the development of a facile and safe sporicidal disinfectant is strongly required. In this study, we have developed an innovative sporicidal disinfection method that employs the combination of an aqueous ethanol solution, visible light irradiation, and a photocatalyst. We successfully produced a sporicidal disinfectant one hundred times as effective as commercially available PAA, while also resolving the hazards and odor problems associated with PAA. The method presented here can potentially be used as a replacement for the general disinfectants employed in the food and health industries.

No MeSH data available.