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Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women

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ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV−ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck®) and cytology. This showed 22/113 (19.5%) of LBC’s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV−ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07–26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV−ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p < 0.05) and smoking (p < 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

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Related in: MedlinePlus

Analysis of the relationship between HTLV-1 infection and life styles factors. The rate of HTLV-1 infection in the smoking group was five times higher than that in non-smoking group. Women with increased numbers of marriages and sexual partners were significantly more likely to have HTLV-1 infections confirming that sexual contact is an important means of HTLV-1 transmission.
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viruses-08-00245-f004: Analysis of the relationship between HTLV-1 infection and life styles factors. The rate of HTLV-1 infection in the smoking group was five times higher than that in non-smoking group. Women with increased numbers of marriages and sexual partners were significantly more likely to have HTLV-1 infections confirming that sexual contact is an important means of HTLV-1 transmission.

Mentions: Analysis of the relationship between HTLV-1 infection and patient’s life style shown in Figure 4 indicated that the rate of HTLV-1 infection in the smoking group was five times higher than that in the non-smoking group. Women with increased numbers of marriages and sex partners were also significantly more likely to have HTLV-1 infections confirming that sexual transmission is an important means of HTLV-1 infection. No significant associations between HTLV-1 infections were found with religion, educational level, occupation, socio-economic status, contraceptive use, intravenous drug use, blood transfusion, genital herpes and either rural or urban residency (see Figure S5).


Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women
Analysis of the relationship between HTLV-1 infection and life styles factors. The rate of HTLV-1 infection in the smoking group was five times higher than that in non-smoking group. Women with increased numbers of marriages and sexual partners were significantly more likely to have HTLV-1 infections confirming that sexual contact is an important means of HTLV-1 transmission.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5035959&req=5

viruses-08-00245-f004: Analysis of the relationship between HTLV-1 infection and life styles factors. The rate of HTLV-1 infection in the smoking group was five times higher than that in non-smoking group. Women with increased numbers of marriages and sexual partners were significantly more likely to have HTLV-1 infections confirming that sexual contact is an important means of HTLV-1 transmission.
Mentions: Analysis of the relationship between HTLV-1 infection and patient’s life style shown in Figure 4 indicated that the rate of HTLV-1 infection in the smoking group was five times higher than that in the non-smoking group. Women with increased numbers of marriages and sex partners were also significantly more likely to have HTLV-1 infections confirming that sexual transmission is an important means of HTLV-1 infection. No significant associations between HTLV-1 infections were found with religion, educational level, occupation, socio-economic status, contraceptive use, intravenous drug use, blood transfusion, genital herpes and either rural or urban residency (see Figure S5).

View Article: PubMed Central - PubMed

ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV−ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck®) and cytology. This showed 22/113 (19.5%) of LBC’s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV−ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07–26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV−ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p < 0.05) and smoking (p < 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

No MeSH data available.


Related in: MedlinePlus