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Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women

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ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV−ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck®) and cytology. This showed 22/113 (19.5%) of LBC’s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV−ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07–26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV−ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p < 0.05) and smoking (p < 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

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Relationship between HTLV-1, HPV, human immunodeficiency virus (HIV) infections and age. There was no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status. However, there was a trend towards a lower HTLV-1 prevalence among women >45 years old when compared to women positive for HPV and HIV.
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viruses-08-00245-f003: Relationship between HTLV-1, HPV, human immunodeficiency virus (HIV) infections and age. There was no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status. However, there was a trend towards a lower HTLV-1 prevalence among women >45 years old when compared to women positive for HPV and HIV.

Mentions: LBC material for this study was obtained from women with an age range of 21 to 52 years and median or mean ages of 35 or 35.2 years, respectively. The median age of HTLV-1 negatives was 35 and HTLV-1 positives 31 years with no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status (Figure 3). Within HTLV-1 positives, no significant difference was found between infection and any of the age groups.


Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women
Relationship between HTLV-1, HPV, human immunodeficiency virus (HIV) infections and age. There was no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status. However, there was a trend towards a lower HTLV-1 prevalence among women >45 years old when compared to women positive for HPV and HIV.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5035959&req=5

viruses-08-00245-f003: Relationship between HTLV-1, HPV, human immunodeficiency virus (HIV) infections and age. There was no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status. However, there was a trend towards a lower HTLV-1 prevalence among women >45 years old when compared to women positive for HPV and HIV.
Mentions: LBC material for this study was obtained from women with an age range of 21 to 52 years and median or mean ages of 35 or 35.2 years, respectively. The median age of HTLV-1 negatives was 35 and HTLV-1 positives 31 years with no significant difference observed between the median age of HTLV-1 positives with respect to either HPV or HIV status (Figure 3). Within HTLV-1 positives, no significant difference was found between infection and any of the age groups.

View Article: PubMed Central - PubMed

ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV−ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck®) and cytology. This showed 22/113 (19.5%) of LBC’s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV−ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07–26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV−ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p < 0.05) and smoking (p < 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

No MeSH data available.


Related in: MedlinePlus