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Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women

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ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV−ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck®) and cytology. This showed 22/113 (19.5%) of LBC’s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV−ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07–26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV−ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p < 0.05) and smoking (p < 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

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Association of HTLV-1 and human papillomavirus (HPV) type 53 infections. HPV type 53 showed a statistically significant association with HTLV-1 infection (p <0.05). None of the other HPV sub-types showed a positive association.
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viruses-08-00245-f002: Association of HTLV-1 and human papillomavirus (HPV) type 53 infections. HPV type 53 showed a statistically significant association with HTLV-1 infection (p <0.05). None of the other HPV sub-types showed a positive association.

Mentions: As described above, 24 subtypes of HPV, including six low-risk (LR) and 18 high-risk (HR) were identified in LBC specimens using the Papillocheck® system. Of the samples analysed, one sample had an invalid result, while 121 (55%, 95%CI: 48.2%–61.7%) had at least one HPV subtype with the highest number of genotypes detected in any one individual being seven, covering 107 HR (88.4%, 95% CI: 81.3%–93.5%) and 14 LR (11.6%, 95% CI: 6.5%–18.7%) HPV subtypes. None of the HPV subtypes covered showed any association with HTLV-1 apart from HPV type 53 where there was a significant positive association (p < 0.05) (Figure 2 and Table 2).


Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women
Association of HTLV-1 and human papillomavirus (HPV) type 53 infections. HPV type 53 showed a statistically significant association with HTLV-1 infection (p <0.05). None of the other HPV sub-types showed a positive association.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5035959&req=5

viruses-08-00245-f002: Association of HTLV-1 and human papillomavirus (HPV) type 53 infections. HPV type 53 showed a statistically significant association with HTLV-1 infection (p <0.05). None of the other HPV sub-types showed a positive association.
Mentions: As described above, 24 subtypes of HPV, including six low-risk (LR) and 18 high-risk (HR) were identified in LBC specimens using the Papillocheck® system. Of the samples analysed, one sample had an invalid result, while 121 (55%, 95%CI: 48.2%–61.7%) had at least one HPV subtype with the highest number of genotypes detected in any one individual being seven, covering 107 HR (88.4%, 95% CI: 81.3%–93.5%) and 14 LR (11.6%, 95% CI: 6.5%–18.7%) HPV subtypes. None of the HPV subtypes covered showed any association with HTLV-1 apart from HPV type 53 where there was a significant positive association (p < 0.05) (Figure 2 and Table 2).

View Article: PubMed Central - PubMed

ABSTRACT

The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV&minus;ve) Kenyan women. Patient sociodemographic details were recorded by structured questionnaire and these data analysed with respect to HIV status, human papillomavirus (HPV) type (Papilocheck&reg;) and cytology. This showed 22/113 (19.5%) of LBC&rsquo;s from HIV+ve patients were positive for HTLV-1 compared to 4/111 (3.6%) of those from HIV&minus;ve women (p = 0.0002; odds ratio (OR) = 6.42 (2.07&ndash;26.56)). Only 1/37 (2.7%) of HIV+ve and none of the 44 HIV&minus;ve ICC samples were positive for HTLV-1. There was also a significant correlation between HTLV-1 infection, numbers of sexual partners (p &lt; 0.05) and smoking (p &lt; 0.01). Using this unique method, these data suggest an unexpectedly high prevalence of HTLV-1 DNA in HIV+ve women in this geographical location. However, the low level of HTLV-1 detected in HIV+ve ICC samples was unexpected and the reasons for this are unclear.

No MeSH data available.


Related in: MedlinePlus