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Humoral and cellular immune responses in mice against secreted and somatic antigens from a C orynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

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ABSTRACT

Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.

Results: The lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.

Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

No MeSH data available.


In vitro cytokine production by murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. a. Interleukin-12 (IL-12). b. Interferon-γ (IFN-γ). c. Interleukin-10 (IL-10). d. Interleukin-4 (IL-4). Results are presented as ρg/mL, and represent the means of two independent experiments using spleen cells retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
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Fig3: In vitro cytokine production by murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. a. Interleukin-12 (IL-12). b. Interferon-γ (IFN-γ). c. Interleukin-10 (IL-10). d. Interleukin-4 (IL-4). Results are presented as ρg/mL, and represent the means of two independent experiments using spleen cells retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05

Mentions: In vitro production of interleukin-12 (IL-12) by spleen cells after stimulation with So or Se antigens is shown in Fig. 3a. Cell stimulated with both antigens had higher IL-12 concentrations at 60 and 120 days post-infection in comparison to controls (p < 0.05).Fig. 3


Humoral and cellular immune responses in mice against secreted and somatic antigens from a C orynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain
In vitro cytokine production by murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. a. Interleukin-12 (IL-12). b. Interferon-γ (IFN-γ). c. Interleukin-10 (IL-10). d. Interleukin-4 (IL-4). Results are presented as ρg/mL, and represent the means of two independent experiments using spleen cells retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5017044&req=5

Fig3: In vitro cytokine production by murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. a. Interleukin-12 (IL-12). b. Interferon-γ (IFN-γ). c. Interleukin-10 (IL-10). d. Interleukin-4 (IL-4). Results are presented as ρg/mL, and represent the means of two independent experiments using spleen cells retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
Mentions: In vitro production of interleukin-12 (IL-12) by spleen cells after stimulation with So or Se antigens is shown in Fig. 3a. Cell stimulated with both antigens had higher IL-12 concentrations at 60 and 120 days post-infection in comparison to controls (p < 0.05).Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.

Results: The lymphocyte proliferation and in vitro production of IFN-&gamma;, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60&nbsp;days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.

Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

No MeSH data available.