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Humoral and cellular immune responses in mice against secreted and somatic antigens from a C orynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

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ABSTRACT

Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.

Results: The lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.

Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

No MeSH data available.


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Proliferation of murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. Results express the stimulation index (μCi) calculated from two independent experiments using splenocytes retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
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Fig2: Proliferation of murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. Results express the stimulation index (μCi) calculated from two independent experiments using splenocytes retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05

Mentions: With respect to spleen cell response to antigenic stimuli, a significant lymphoproliferative response, expressed as SI, was observed after stimulation with secreted antigen (Se) (p < 0.05) at 60 days post-infection in comparison to 7 and 30 days (Fig. 2). Stimulation with Se provoked a significant difference in SI in comparison to So at 60 days after inoculation.Fig. 2


Humoral and cellular immune responses in mice against secreted and somatic antigens from a C orynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain
Proliferation of murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. Results express the stimulation index (μCi) calculated from two independent experiments using splenocytes retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5017044&req=5

Fig2: Proliferation of murine spleen cells stimulated with somatic (So) and secreted (Se) antigens. Results express the stimulation index (μCi) calculated from two independent experiments using splenocytes retrieved from five non-infected (control) and five infected (inoculated) animals from each group. Data were analyzed by ANOVA and Tukey post-hoc tests; *P < 0.05
Mentions: With respect to spleen cell response to antigenic stimuli, a significant lymphoproliferative response, expressed as SI, was observed after stimulation with secreted antigen (Se) (p < 0.05) at 60 days post-infection in comparison to 7 and 30 days (Fig. 2). Stimulation with Se provoked a significant difference in SI in comparison to So at 60 days after inoculation.Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.

Results: The lymphocyte proliferation and in vitro production of IFN-&gamma;, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60&nbsp;days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.

Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

No MeSH data available.


Related in: MedlinePlus