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Treatment with Rhus tripartita extract curtails isoproterenol-elicited cardiotoxicity and oxidative stress in rats

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of plant-derived nutraceuticals and crude drugs in traditional medicine is widely believed to confer beneficial effects in thwarting the progression of cardiovascular diseases. Rhus tripartita (family Anacardiaceae) has been traditionally used to treat a wide range of ailments.

Methods: In the present study we investigated the protective effects of an alcoholic extract of the stem part of Rhus tripartita male genotype (RTSM) on experimentally induced myocardial injury in rats. To this end, cardiac injury was induced by administration of isoproterenol (ISO) and serum enzyme markers, lipid profiles and cardiac tissue redox status were determined following RTSM treatment (250 and 500 mg/kg).

1234566: As a result, RTSM treatment significantly mitigated ISO-triggered upregulation of cardiac-specific markers of injury creatine kinase and lactate dehydrogenase. RTSM treatment significantly attenuated ISO-induced increase in serum cholesterol and triglycerides as well alterations in serum lipoproteins. Determination of oxidative balance showed that RTSM treatment significantly blunted ISO-induced increase in malondialdehyde and decrease in nonprotein sulfhydryl in cardiac tissue. Six compounds were isolated and identified as gallocatechin , taxifolin , myricetin-3-O-β-glucoside , catechin , epicatechin , and 3′,8-binaringenin . Compound was isolated for the first time from the stem part of Rhus tripartita. Furthermore, RTSM treatment enhanced the survival fraction of cardiac cells exposed to oxidative stress in vitro.

Conclusion: We conclude that the antioxidant properties of RTSM treatment underpin its cardioprotective pharmacological effects, thus, providing biological evidence for the treatment of cardiovascular diseases using Rhus tripartita in indigenous medicine.

No MeSH data available.


Related in: MedlinePlus

Photomicrography of cardiac tissue of rats showing the effect of RTSM. a Normal (control group) showing no significant pathology. H.& E. 400x; b Cardiac tissue showing myocardial necrosis with lymphohistiocytic, reaction around the necrotic myocardial fibers in rats myocardial cells treated with ISO only. H. & E. 600x; c RTSM (250 mg/kg) plus ISO pretreatment showing slightly disorganized myocardial fibres with focal chronic inflammation. H. & E. 600x. d RTSM (500 mg/kg) plus ISO pretreated group showing a small focus of myocardial fibres tic reaction. H. & E. 600x
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Fig5: Photomicrography of cardiac tissue of rats showing the effect of RTSM. a Normal (control group) showing no significant pathology. H.& E. 400x; b Cardiac tissue showing myocardial necrosis with lymphohistiocytic, reaction around the necrotic myocardial fibers in rats myocardial cells treated with ISO only. H. & E. 600x; c RTSM (250 mg/kg) plus ISO pretreatment showing slightly disorganized myocardial fibres with focal chronic inflammation. H. & E. 600x. d RTSM (500 mg/kg) plus ISO pretreated group showing a small focus of myocardial fibres tic reaction. H. & E. 600x

Mentions: The histology of rat cardiac tissues showed that ISO-elicited inflammatory lesions led to structure disorders of muscle fibers as well as infiltration of acute inflammatory cells, including extravasation of red blood cells (Fig. 5a and b). The other changes observed were interstitial edema and the appearance of vacuoles in ISO alone treated rats. RTSM treatment mitigated ISO-induced inflammatory changes in cardiac tissues, indicating that RTSM efficiently cured the tissue lesions in a dose-dependent manner (Fig. 5c and d).


Treatment with Rhus tripartita extract curtails isoproterenol-elicited cardiotoxicity and oxidative stress in rats
Photomicrography of cardiac tissue of rats showing the effect of RTSM. a Normal (control group) showing no significant pathology. H.& E. 400x; b Cardiac tissue showing myocardial necrosis with lymphohistiocytic, reaction around the necrotic myocardial fibers in rats myocardial cells treated with ISO only. H. & E. 600x; c RTSM (250 mg/kg) plus ISO pretreatment showing slightly disorganized myocardial fibres with focal chronic inflammation. H. & E. 600x. d RTSM (500 mg/kg) plus ISO pretreated group showing a small focus of myocardial fibres tic reaction. H. & E. 600x
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5017009&req=5

Fig5: Photomicrography of cardiac tissue of rats showing the effect of RTSM. a Normal (control group) showing no significant pathology. H.& E. 400x; b Cardiac tissue showing myocardial necrosis with lymphohistiocytic, reaction around the necrotic myocardial fibers in rats myocardial cells treated with ISO only. H. & E. 600x; c RTSM (250 mg/kg) plus ISO pretreatment showing slightly disorganized myocardial fibres with focal chronic inflammation. H. & E. 600x. d RTSM (500 mg/kg) plus ISO pretreated group showing a small focus of myocardial fibres tic reaction. H. & E. 600x
Mentions: The histology of rat cardiac tissues showed that ISO-elicited inflammatory lesions led to structure disorders of muscle fibers as well as infiltration of acute inflammatory cells, including extravasation of red blood cells (Fig. 5a and b). The other changes observed were interstitial edema and the appearance of vacuoles in ISO alone treated rats. RTSM treatment mitigated ISO-induced inflammatory changes in cardiac tissues, indicating that RTSM efficiently cured the tissue lesions in a dose-dependent manner (Fig. 5c and d).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of plant-derived nutraceuticals and crude drugs in traditional medicine is widely believed to confer beneficial effects in thwarting the progression of cardiovascular diseases. Rhus tripartita (family Anacardiaceae) has been traditionally used to treat a wide range of ailments.

Methods: In the present study we investigated the protective effects of an alcoholic extract of the stem part of Rhus tripartita male genotype (RTSM) on experimentally induced myocardial injury in rats. To this end, cardiac injury was induced by administration of isoproterenol (ISO) and serum enzyme markers, lipid profiles and cardiac tissue redox status were determined following RTSM treatment (250 and 500 mg/kg).

1234566: As a result, RTSM treatment significantly mitigated ISO-triggered upregulation of cardiac-specific markers of injury creatine kinase and lactate dehydrogenase. RTSM treatment significantly attenuated ISO-induced increase in serum cholesterol and triglycerides as well alterations in serum lipoproteins. Determination of oxidative balance showed that RTSM treatment significantly blunted ISO-induced increase in malondialdehyde and decrease in nonprotein sulfhydryl in cardiac tissue. Six compounds were isolated and identified as gallocatechin , taxifolin , myricetin-3-O-β-glucoside , catechin , epicatechin , and 3′,8-binaringenin . Compound was isolated for the first time from the stem part of Rhus tripartita. Furthermore, RTSM treatment enhanced the survival fraction of cardiac cells exposed to oxidative stress in vitro.

Conclusion: We conclude that the antioxidant properties of RTSM treatment underpin its cardioprotective pharmacological effects, thus, providing biological evidence for the treatment of cardiovascular diseases using Rhus tripartita in indigenous medicine.

No MeSH data available.


Related in: MedlinePlus