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Complete genome sequence of Desulfurivibrio alkaliphilus strain AHT2 T , a haloalkaliphilic sulfidogen from Egyptian hypersaline alkaline lakes

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ABSTRACT

Desulfurivibrio alkaliphilus strain AHT2T is a strictly anaerobic sulfidogenic haloalkaliphile isolated from a composite sediment sample of eight hypersaline alkaline lakes in the Wadi al Natrun valley in the Egyptian Libyan Desert. D. alkaliphilus AHT2T is Gram-negative and belongs to the family Desulfobulbaceae within the Deltaproteobacteria. Here we report its genome sequence, which contains a 3.10 Mbp chromosome. D. alkaliphilus AHT2T is adapted to survive under highly alkaline and moderately saline conditions and therefore, is relevant to the biotechnology industry and life under extreme conditions. For these reasons, D. alkaliphilus AHT2T was sequenced by the DOE Joint Genome Institute as part of the Community Science Program.

No MeSH data available.


The sodium dependent NADH ubiquinone oxidoreductase (nqr) gene cluster. Vibrio alginolyticus ATCC 17749T [33, 48] was used as a reference for the delta proteobacterium sp. MLMS-1 and D. alkaliphilus AHT2T gene clusters
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Fig5: The sodium dependent NADH ubiquinone oxidoreductase (nqr) gene cluster. Vibrio alginolyticus ATCC 17749T [33, 48] was used as a reference for the delta proteobacterium sp. MLMS-1 and D. alkaliphilus AHT2T gene clusters

Mentions: There are several adaptations that haloalkaliphiles can use to survive in the haloalkaline environment: bioenergetic adaptations, structural membrane adaptations and the use of osmoprotectants to retain osmotic balance [1]. The genome of D. alkaliphilus AHT2T contains a voltage gated sodium channel gene ncbA (DaAHT2_0077) and the electrogenic sodium/proton antiporter mrpBCDEFG operon (DaAHT2_2362 to DaAHT2_2357). The nqr operon encodes a sodium pumping NADH: quinone oxidoreductase (alternative to H+-pumping conventional NADH-quionone oxidoreductases) that shuttles electrons from NADH to ubiquinone [33, 34]. The D. alkaliphilus AHT2T genome contains the first account of the nqr operon in anaerobic haloalkaliphiles [35, 36]. The locus tags of the nqr gene cluster nqrA-nqrF in D. alkaliphilus AHT2T are DaAHT2_0042 – DaAHT2_0047, and we also found this cluster in D. alkaliphilus AHT2T’s closest sequenced relative delta proteobacterium sp. MLMS-1 (mldDRAFT_0493-0498) (Fig. 5). The D. alkaliphilus AHT2T genome does not contain genes for the synthesis of ectoine or betaine, which function as common osmoprotectants in haloalkaliphiles, but it does have a choline/betaine transporter (DaAHT2_1056).Fig. 5


Complete genome sequence of Desulfurivibrio alkaliphilus strain AHT2 T , a haloalkaliphilic sulfidogen from Egyptian hypersaline alkaline lakes
The sodium dependent NADH ubiquinone oxidoreductase (nqr) gene cluster. Vibrio alginolyticus ATCC 17749T [33, 48] was used as a reference for the delta proteobacterium sp. MLMS-1 and D. alkaliphilus AHT2T gene clusters
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5016858&req=5

Fig5: The sodium dependent NADH ubiquinone oxidoreductase (nqr) gene cluster. Vibrio alginolyticus ATCC 17749T [33, 48] was used as a reference for the delta proteobacterium sp. MLMS-1 and D. alkaliphilus AHT2T gene clusters
Mentions: There are several adaptations that haloalkaliphiles can use to survive in the haloalkaline environment: bioenergetic adaptations, structural membrane adaptations and the use of osmoprotectants to retain osmotic balance [1]. The genome of D. alkaliphilus AHT2T contains a voltage gated sodium channel gene ncbA (DaAHT2_0077) and the electrogenic sodium/proton antiporter mrpBCDEFG operon (DaAHT2_2362 to DaAHT2_2357). The nqr operon encodes a sodium pumping NADH: quinone oxidoreductase (alternative to H+-pumping conventional NADH-quionone oxidoreductases) that shuttles electrons from NADH to ubiquinone [33, 34]. The D. alkaliphilus AHT2T genome contains the first account of the nqr operon in anaerobic haloalkaliphiles [35, 36]. The locus tags of the nqr gene cluster nqrA-nqrF in D. alkaliphilus AHT2T are DaAHT2_0042 – DaAHT2_0047, and we also found this cluster in D. alkaliphilus AHT2T’s closest sequenced relative delta proteobacterium sp. MLMS-1 (mldDRAFT_0493-0498) (Fig. 5). The D. alkaliphilus AHT2T genome does not contain genes for the synthesis of ectoine or betaine, which function as common osmoprotectants in haloalkaliphiles, but it does have a choline/betaine transporter (DaAHT2_1056).Fig. 5

View Article: PubMed Central - PubMed

ABSTRACT

Desulfurivibrio alkaliphilus strain AHT2T is a strictly anaerobic sulfidogenic haloalkaliphile isolated from a composite sediment sample of eight hypersaline alkaline lakes in the Wadi al Natrun valley in the Egyptian Libyan Desert. D. alkaliphilus AHT2T is Gram-negative and belongs to the family Desulfobulbaceae within the Deltaproteobacteria. Here we report its genome sequence, which contains a 3.10 Mbp chromosome. D. alkaliphilus AHT2T is adapted to survive under highly alkaline and moderately saline conditions and therefore, is relevant to the biotechnology industry and life under extreme conditions. For these reasons, D. alkaliphilus AHT2T was sequenced by the DOE Joint Genome Institute as part of the Community Science Program.

No MeSH data available.