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Complete genome sequence of Desulfurivibrio alkaliphilus strain AHT2 T , a haloalkaliphilic sulfidogen from Egyptian hypersaline alkaline lakes

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ABSTRACT

Desulfurivibrio alkaliphilus strain AHT2T is a strictly anaerobic sulfidogenic haloalkaliphile isolated from a composite sediment sample of eight hypersaline alkaline lakes in the Wadi al Natrun valley in the Egyptian Libyan Desert. D. alkaliphilus AHT2T is Gram-negative and belongs to the family Desulfobulbaceae within the Deltaproteobacteria. Here we report its genome sequence, which contains a 3.10 Mbp chromosome. D. alkaliphilus AHT2T is adapted to survive under highly alkaline and moderately saline conditions and therefore, is relevant to the biotechnology industry and life under extreme conditions. For these reasons, D. alkaliphilus AHT2T was sequenced by the DOE Joint Genome Institute as part of the Community Science Program.

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D. alkaliphilus AHT2T Wood-Ljungdahl pathway genes, including the acs gene cluster, based on delta proteobacterium sp. MLMS-1 [23]. The gene locus tags are depicted beneath the illustrated gene representations
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Fig3: D. alkaliphilus AHT2T Wood-Ljungdahl pathway genes, including the acs gene cluster, based on delta proteobacterium sp. MLMS-1 [23]. The gene locus tags are depicted beneath the illustrated gene representations

Mentions: In order to grow chemolithoautotrophically, D. alkaliphilus AHT2T assimilates inorganic carbon from the environment. The genome of D. alkaliphilus AHT2T contains the key genes necessary for the WL pathway, a mode of carbon fixation from CO2, which can run in the reductive and oxidative direction [22]. In the reductive direction, carbon is fixed from inorganic CO2 to cell material. The WL pathway functions in this direction in many representatives of sulfate-reducing bacteria within the Deltaproteobacteria. Some organisms may couple the reverse, or oxidative, direction to sulfate reduction. The WL gene clusters have previously been defined for delta proteobacterium sp. MLMS-1 from Mono Lake [23], the closest sequenced relative of D. alkaliphilus AHT2T (Fig. 2). Here we identified the WL genes necessary for carbon fixation by comparing the corresponding delta proteobacterium sp. MLMS-1 gene clusters to those present in D. alkaliphilus AHT2T using the JGI IMG database (Fig. 3). The first step in the reductive pathway is the reduction of CO2 to formate, by formate dehydrogenase (DaAHT2_0823 and an accessory protein DaAHT2_0820). This is followed by formyl-THF synthetase (DaAHT2_0837) and a methylene-THF dehydrogenase/cyclohydrolase (DaAHT2_0828) and a methylene-THF reductase (DaAHT2_0827). The acs gene cluster is necessary for the carbonyl branch of the reaction [22], which starts with the reduction of CO2 to carbon monoxide by a carbon monoxide dehydrogenase (DaAHT2_0826). In the last step, the products of the carbonyl and methyl branch are combined to form the product acetyl-CoA, by a CO dehydrogenase/acetyl-CoA synthase complex (DaAHT2_0825 and DaAHT2_0824). The end product of the WL cycle is typically acetate, however, the genes needed to convert acetyl-CoA to the end product acetate are absent in the D. alkaliphilus AHT2T genome, resulting in acetyl CoA being the carbon end product which can be incorporated into biomass.Fig. 3


Complete genome sequence of Desulfurivibrio alkaliphilus strain AHT2 T , a haloalkaliphilic sulfidogen from Egyptian hypersaline alkaline lakes
D. alkaliphilus AHT2T Wood-Ljungdahl pathway genes, including the acs gene cluster, based on delta proteobacterium sp. MLMS-1 [23]. The gene locus tags are depicted beneath the illustrated gene representations
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5016858&req=5

Fig3: D. alkaliphilus AHT2T Wood-Ljungdahl pathway genes, including the acs gene cluster, based on delta proteobacterium sp. MLMS-1 [23]. The gene locus tags are depicted beneath the illustrated gene representations
Mentions: In order to grow chemolithoautotrophically, D. alkaliphilus AHT2T assimilates inorganic carbon from the environment. The genome of D. alkaliphilus AHT2T contains the key genes necessary for the WL pathway, a mode of carbon fixation from CO2, which can run in the reductive and oxidative direction [22]. In the reductive direction, carbon is fixed from inorganic CO2 to cell material. The WL pathway functions in this direction in many representatives of sulfate-reducing bacteria within the Deltaproteobacteria. Some organisms may couple the reverse, or oxidative, direction to sulfate reduction. The WL gene clusters have previously been defined for delta proteobacterium sp. MLMS-1 from Mono Lake [23], the closest sequenced relative of D. alkaliphilus AHT2T (Fig. 2). Here we identified the WL genes necessary for carbon fixation by comparing the corresponding delta proteobacterium sp. MLMS-1 gene clusters to those present in D. alkaliphilus AHT2T using the JGI IMG database (Fig. 3). The first step in the reductive pathway is the reduction of CO2 to formate, by formate dehydrogenase (DaAHT2_0823 and an accessory protein DaAHT2_0820). This is followed by formyl-THF synthetase (DaAHT2_0837) and a methylene-THF dehydrogenase/cyclohydrolase (DaAHT2_0828) and a methylene-THF reductase (DaAHT2_0827). The acs gene cluster is necessary for the carbonyl branch of the reaction [22], which starts with the reduction of CO2 to carbon monoxide by a carbon monoxide dehydrogenase (DaAHT2_0826). In the last step, the products of the carbonyl and methyl branch are combined to form the product acetyl-CoA, by a CO dehydrogenase/acetyl-CoA synthase complex (DaAHT2_0825 and DaAHT2_0824). The end product of the WL cycle is typically acetate, however, the genes needed to convert acetyl-CoA to the end product acetate are absent in the D. alkaliphilus AHT2T genome, resulting in acetyl CoA being the carbon end product which can be incorporated into biomass.Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Desulfurivibrio alkaliphilus strain AHT2T is a strictly anaerobic sulfidogenic haloalkaliphile isolated from a composite sediment sample of eight hypersaline alkaline lakes in the Wadi al Natrun valley in the Egyptian Libyan Desert. D. alkaliphilus AHT2T is Gram-negative and belongs to the family Desulfobulbaceae within the Deltaproteobacteria. Here we report its genome sequence, which contains a 3.10 Mbp chromosome. D. alkaliphilus AHT2T is adapted to survive under highly alkaline and moderately saline conditions and therefore, is relevant to the biotechnology industry and life under extreme conditions. For these reasons, D. alkaliphilus AHT2T was sequenced by the DOE Joint Genome Institute as part of the Community Science Program.

No MeSH data available.


Related in: MedlinePlus