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Raman-based detection of hydroxyethyl starch in kidney allograft biopsies as a potential marker of allograft quality in kidney transplant recipients

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ABSTRACT

In brain-dead donor resuscitation, hydroxyethyl starch (HES) use has been associated with presence of osmotic-nephrosis-like lesions in kidney transplant recipients. Our aim was to determine whether the presence of HES in protocol renal graft biopsies at three months (M3) after transplantation is associated with renal graft quality. According to the HES administered to the donor during the procurement procedure, two groups of patients were defined according graft exposition to HES: HES group, (N = 20) and control group (N = 6). Detection and relative quantification of HES was performed by Raman spectroscopy microimaging on M3 protocol renal graft biopsies. Statistical analyses were used to investigate the association between Raman data and graft characteristics. HES spectral signal was revealed negative in the control group, whereas it was positive in 40% of biopsies from the HES group. In the HES group, a stronger HES signal was associated with a lower risk of graft failure measured by the Kidney Donor Risk Index (KDRI) and was correlated with the allograft kidney function. Thus, HES accumulation in donor kidney, as probed by Raman biophotonic technique, is correlated with the quality of donor kidney and consequently the graft renal function and graft survival.

No MeSH data available.


Flow chart of the study design.
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f1: Flow chart of the study design.

Mentions: In our study, 26 renal transplantations were analysed (Fig. 1): 20 cases for which HES 130/0.4 (VOLUVEN; FRESENIUS-KABI) had been administered in the 72 hours prior to organ retrieval (HES group) and 6 transplantations where no colloid compounds were used (control group). The clinic-demographic characteristics of the brain-dead kidney donors and transplant recipients are shown in Table 1. Biopsies corresponding to these 26 M3 grafts were examined by Raman imaging.


Raman-based detection of hydroxyethyl starch in kidney allograft biopsies as a potential marker of allograft quality in kidney transplant recipients
Flow chart of the study design.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5016807&req=5

f1: Flow chart of the study design.
Mentions: In our study, 26 renal transplantations were analysed (Fig. 1): 20 cases for which HES 130/0.4 (VOLUVEN; FRESENIUS-KABI) had been administered in the 72 hours prior to organ retrieval (HES group) and 6 transplantations where no colloid compounds were used (control group). The clinic-demographic characteristics of the brain-dead kidney donors and transplant recipients are shown in Table 1. Biopsies corresponding to these 26 M3 grafts were examined by Raman imaging.

View Article: PubMed Central - PubMed

ABSTRACT

In brain-dead donor resuscitation, hydroxyethyl starch (HES) use has been associated with presence of osmotic-nephrosis-like lesions in kidney transplant recipients. Our aim was to determine whether the presence of HES in protocol renal graft biopsies at three months (M3) after transplantation is associated with renal graft quality. According to the HES administered to the donor during the procurement procedure, two groups of patients were defined according graft exposition to HES: HES group, (N = 20) and control group (N = 6). Detection and relative quantification of HES was performed by Raman spectroscopy microimaging on M3 protocol renal graft biopsies. Statistical analyses were used to investigate the association between Raman data and graft characteristics. HES spectral signal was revealed negative in the control group, whereas it was positive in 40% of biopsies from the HES group. In the HES group, a stronger HES signal was associated with a lower risk of graft failure measured by the Kidney Donor Risk Index (KDRI) and was correlated with the allograft kidney function. Thus, HES accumulation in donor kidney, as probed by Raman biophotonic technique, is correlated with the quality of donor kidney and consequently the graft renal function and graft survival.

No MeSH data available.