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Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China

View Article: PubMed Central - PubMed

ABSTRACT

The anthracnose caused by Colletotrichum species is an important disease that primarily causes fruit rot in pepper. Eighty-eight strains representing seven species of Colletotrichum were obtained from rotten pepper fruits in Sichuan Province, China, and characterized according to morphology and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequence. Fifty-two strains were chosen for identification by phylogenetic analyses of multi-locus sequences, including the nuclear ribosomal internal transcribed spacer (ITS) region and the β-tubulin (TUB2), actin (ACT), calmodulin (CAL) and GAPDH genes. Based on the combined datasets, the 88 strains were identified as Colletotrichum gloeosporioides, C. siamense, C. fructicola, C. truncatum, C. scovillei, and C. brevisporum, and one new species was detected, described as Colletotrichum sichuanensis. Notably, C. siamense and C. scovillei were recorded for the first time as the causes of anthracnose in peppers in China. In addition, with the exception of C. truncatum, this is the first report of all of the other Colletotrichum species studied in pepper from Sichuan. The fungal species were all non-host-specific, as the isolates were able to infect not only Capsicum spp. but also Pyrus pyrifolia in pathogenicity tests. These findings suggest that the fungal species associated with anthracnose in pepper may inoculate other hosts as initial inoculum.

No MeSH data available.


Phylogram generated from maximum parsimony analysis based on alignment of ITS, TUB2, ACT, GADPH and CAL gene sequences, showing the phylogenetic relationships of Colletotrichum species causing anthracnose disease in Capsicum annuum from Sichuan Province, China.Parsimony bootstrap values of more than 50% are shown at the nodes. Isolates from this study are shown in bold. The tree is rooted with Monilochaetes infuscans.
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f4: Phylogram generated from maximum parsimony analysis based on alignment of ITS, TUB2, ACT, GADPH and CAL gene sequences, showing the phylogenetic relationships of Colletotrichum species causing anthracnose disease in Capsicum annuum from Sichuan Province, China.Parsimony bootstrap values of more than 50% are shown at the nodes. Isolates from this study are shown in bold. The tree is rooted with Monilochaetes infuscans.

Mentions: Multi-locus phylogenetic analysis was conducted among 87 strains, with Monilochaetes infuscans (CBS 869.96) used as an outgroup (Table 3). The dataset for five genes (ITS, TUB2, ACT, GAPDH and CAL) contained 2,155 characters, including alignment gaps, of which 997 characters were parsimony-informative, 321 were parsimony-uninformative, and 837 were constant. This parsimony analysis resulted in the most parsimonious tree (TL = 2800, CI = 0.7257, RI = 0.9541, RC = 0.6924, and HI = 0.2743). The phylogram showed that the 52 pepper anthracnose isolates belonged to seven distinct clades. The isolates from Group 2 clustered with C. fructicola, those from Group 3 clustered with C. truncatum, those from Group 4 clustered with C. scovillei, and those from Group 5 clustered with C. brevisporum. The Group 1 isolates grouped with two clades; 4 isolates clustered with C. gloeosporioides, and the remaining isolates clustered with C. siamense (Fig. 4). After combining two phylograms (Figs 3 and 4), 8 and 16 strains were found to belong to C. gloeosporioides sensu stricto and C. siamense, respectively. The isolates from Group 6 were from an unknown species (Colletotrichum sp.). The submission number of the sequence alignment in TreeBASE is 18832.


Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China
Phylogram generated from maximum parsimony analysis based on alignment of ITS, TUB2, ACT, GADPH and CAL gene sequences, showing the phylogenetic relationships of Colletotrichum species causing anthracnose disease in Capsicum annuum from Sichuan Province, China.Parsimony bootstrap values of more than 50% are shown at the nodes. Isolates from this study are shown in bold. The tree is rooted with Monilochaetes infuscans.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5016793&req=5

f4: Phylogram generated from maximum parsimony analysis based on alignment of ITS, TUB2, ACT, GADPH and CAL gene sequences, showing the phylogenetic relationships of Colletotrichum species causing anthracnose disease in Capsicum annuum from Sichuan Province, China.Parsimony bootstrap values of more than 50% are shown at the nodes. Isolates from this study are shown in bold. The tree is rooted with Monilochaetes infuscans.
Mentions: Multi-locus phylogenetic analysis was conducted among 87 strains, with Monilochaetes infuscans (CBS 869.96) used as an outgroup (Table 3). The dataset for five genes (ITS, TUB2, ACT, GAPDH and CAL) contained 2,155 characters, including alignment gaps, of which 997 characters were parsimony-informative, 321 were parsimony-uninformative, and 837 were constant. This parsimony analysis resulted in the most parsimonious tree (TL = 2800, CI = 0.7257, RI = 0.9541, RC = 0.6924, and HI = 0.2743). The phylogram showed that the 52 pepper anthracnose isolates belonged to seven distinct clades. The isolates from Group 2 clustered with C. fructicola, those from Group 3 clustered with C. truncatum, those from Group 4 clustered with C. scovillei, and those from Group 5 clustered with C. brevisporum. The Group 1 isolates grouped with two clades; 4 isolates clustered with C. gloeosporioides, and the remaining isolates clustered with C. siamense (Fig. 4). After combining two phylograms (Figs 3 and 4), 8 and 16 strains were found to belong to C. gloeosporioides sensu stricto and C. siamense, respectively. The isolates from Group 6 were from an unknown species (Colletotrichum sp.). The submission number of the sequence alignment in TreeBASE is 18832.

View Article: PubMed Central - PubMed

ABSTRACT

The anthracnose caused by Colletotrichum species is an important disease that primarily causes fruit rot in pepper. Eighty-eight strains representing seven species of Colletotrichum were obtained from rotten pepper fruits in Sichuan Province, China, and characterized according to morphology and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequence. Fifty-two strains were chosen for identification by phylogenetic analyses of multi-locus sequences, including the nuclear ribosomal internal transcribed spacer (ITS) region and the β-tubulin (TUB2), actin (ACT), calmodulin (CAL) and GAPDH genes. Based on the combined datasets, the 88 strains were identified as Colletotrichum gloeosporioides, C. siamense, C. fructicola, C. truncatum, C. scovillei, and C. brevisporum, and one new species was detected, described as Colletotrichum sichuanensis. Notably, C. siamense and C. scovillei were recorded for the first time as the causes of anthracnose in peppers in China. In addition, with the exception of C. truncatum, this is the first report of all of the other Colletotrichum species studied in pepper from Sichuan. The fungal species were all non-host-specific, as the isolates were able to infect not only Capsicum spp. but also Pyrus pyrifolia in pathogenicity tests. These findings suggest that the fungal species associated with anthracnose in pepper may inoculate other hosts as initial inoculum.

No MeSH data available.