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Effect of a local, one time, low-dose injection of zoledronic acid on titanium implant osseointegration in ovariectomized rats

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Local application of bisphosphonates has been proven to be safer than systemic administration to promote implant fixation. The objective of this study was to introduce such a simple, convenient and efficient method to enhance titanium (Ti) implant osseointegration in ovariectomized (OVX) rats.

Material and methods: Twenty female Sprague-Dawley rats sequentially underwent bilateral ovariectomy and tibia implantation, and injection of 30 µg/implant zoledronic acid (ZOL) at the site of implantation was performed. At the end of the study, the tibiae, mandibles, femurs and vertebrae were harvested for dual energy X-ray absorptiometry, histology and micro-computed tomography examination.

Results: Ovariectomized rats showed poor bone density, bone mass and trabecular microstructure. OVX + ZOL rats were characterized by significantly improved peri-implant bone area (1.72-fold), bone contact (2.30-fold), bone mineral density (1.57-fold) and bone mineral content (1.67-fold), as well as moderately increased bone volume to total volume ratio (1.34-fold), percentage osteointegration (1.54-fold), connectivity density (1.45-fold), and trabecular number (1.43-fold), but decreased trabecular separation (57.69%) when compared with the control levels (p < 0.05). No histological signs of jaw osteonecrosis were observed in the rats treated with ZOL, and there was no significant difference between the OVX group and OVX + ZOL group in the bone mass of the mandible, femur and 5th lumbar vertebra (p > 0.05). In addition, the overproduction of osteoporosis-induced advanced glycation end-products (AGEs) was completely prevented by local treatment with 30 µg/implant ZOL.

Conclusions: A local, one time, low-dose injection of ZOL at the site of implantation is able to promote the osseointegration of Ti implants following postmenopausal osteoporosis, and this action may be partly mediated by inhibition of the osteoporosis-induced AGE overproduction in the bone marrow.

No MeSH data available.


Representative 3D micro-CT images of the proximal tibia with titanium implants 3 months after implantation
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Figure 0003: Representative 3D micro-CT images of the proximal tibia with titanium implants 3 months after implantation

Mentions: The high-resolution 3D images obtained from micro-CT clearly confirmed the results of histological analysis. Three months after implantation, local injection of 30 µg/implant ZOL exhibited a significant effect in promoting the peri-implant 3D bone volume (Figure 3). The OVX rats had lower values of BV/TV (22.61 ±2.46%), %OI (26.01 ±3.23%), Conn.D (24.34 ±3.42 mm–3), and Tb.N (2.93 ±0.20 mm–1), with little bone apposition around the Ti implants. OVX + ZOL rats were characterized by moderately increased BV/TV (1.34-fold),%OI (1.54-fold), Conn.D (1.45-fold), and Tb.N (1.43-fold), but decreased Tb.Sp (57.69%) when compared with the control levels. However, by contrast, no significant difference was found in the trabecular thickness between the two groups (p > 0.05; Figure 4).


Effect of a local, one time, low-dose injection of zoledronic acid on titanium implant osseointegration in ovariectomized rats
Representative 3D micro-CT images of the proximal tibia with titanium implants 3 months after implantation
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5016583&req=5

Figure 0003: Representative 3D micro-CT images of the proximal tibia with titanium implants 3 months after implantation
Mentions: The high-resolution 3D images obtained from micro-CT clearly confirmed the results of histological analysis. Three months after implantation, local injection of 30 µg/implant ZOL exhibited a significant effect in promoting the peri-implant 3D bone volume (Figure 3). The OVX rats had lower values of BV/TV (22.61 ±2.46%), %OI (26.01 ±3.23%), Conn.D (24.34 ±3.42 mm–3), and Tb.N (2.93 ±0.20 mm–1), with little bone apposition around the Ti implants. OVX + ZOL rats were characterized by moderately increased BV/TV (1.34-fold),%OI (1.54-fold), Conn.D (1.45-fold), and Tb.N (1.43-fold), but decreased Tb.Sp (57.69%) when compared with the control levels. However, by contrast, no significant difference was found in the trabecular thickness between the two groups (p > 0.05; Figure 4).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Local application of bisphosphonates has been proven to be safer than systemic administration to promote implant fixation. The objective of this study was to introduce such a simple, convenient and efficient method to enhance titanium (Ti) implant osseointegration in ovariectomized (OVX) rats.

Material and methods: Twenty female Sprague-Dawley rats sequentially underwent bilateral ovariectomy and tibia implantation, and injection of 30 µg/implant zoledronic acid (ZOL) at the site of implantation was performed. At the end of the study, the tibiae, mandibles, femurs and vertebrae were harvested for dual energy X-ray absorptiometry, histology and micro-computed tomography examination.

Results: Ovariectomized rats showed poor bone density, bone mass and trabecular microstructure. OVX + ZOL rats were characterized by significantly improved peri-implant bone area (1.72-fold), bone contact (2.30-fold), bone mineral density (1.57-fold) and bone mineral content (1.67-fold), as well as moderately increased bone volume to total volume ratio (1.34-fold), percentage osteointegration (1.54-fold), connectivity density (1.45-fold), and trabecular number (1.43-fold), but decreased trabecular separation (57.69%) when compared with the control levels (p < 0.05). No histological signs of jaw osteonecrosis were observed in the rats treated with ZOL, and there was no significant difference between the OVX group and OVX + ZOL group in the bone mass of the mandible, femur and 5th lumbar vertebra (p > 0.05). In addition, the overproduction of osteoporosis-induced advanced glycation end-products (AGEs) was completely prevented by local treatment with 30 µg/implant ZOL.

Conclusions: A local, one time, low-dose injection of ZOL at the site of implantation is able to promote the osseointegration of Ti implants following postmenopausal osteoporosis, and this action may be partly mediated by inhibition of the osteoporosis-induced AGE overproduction in the bone marrow.

No MeSH data available.