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The effects of treadmill exercise on penicillin-induced epileptiform activity

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: The aim of this study was to evaluate the effects of short-, moderate- and long-duration treadmill exercise (15, 30 and 60 min) on the mean frequency and amplitude of penicillin-induced epileptiform activity in rats.

Material and methods: In this study, 32 rats were assigned to 15, 30, and 60 min running exercise groups and the control group, each consisting of 8 rats. According to the specified protocol, the rats were submitted to running exercises at the same hour of each day for 90 days. After the exercise program, the rats were administered (500 IU/2.5 µl) of penicillin into the left cortex by the microinjection method. An electrocorticogram (ECoG) recording was performed for 3 h using a data acquisition system. The frequency and the amplitude of the recordings were analyzed.

Results: Short-duration treadmill exercise (15 min) caused a decrease in the frequency of penicillin-induced epileptiform activity at 70 min after penicillin injection (p < 0.001). The mean frequency of epileptiform activity decreased at 90 min after penicillin injection in the 30 and 60 min treadmill exercise groups (p < 0.01). The mean amplitude of epileptiform activity was not changed in any of the exercise groups compared to the control (p > 0.05).

Conclusions: The results of the present study demonstrate for the first time that short-, moderate- and long-duration treadmill exercises decreased the frequency of penicillin-induced epileptiform activity. These findings may contribute to improving the quality of life in epileptic patients.

No MeSH data available.


A – The intracortical injection of penicillin (500 IU/2.5 µl) induced epileptiform activity on ECoG. B – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 70 min after penicillin injection in the 15 min treadmill exercise group (treadmill exercise 15 min/day, for 90 days). C – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 30 min treadmill exercise group (treadmill exercise 30 min/day, for 90 days). D – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 60 min treadmill exercise group (treadmill exercise 60 min/day, for 90 days). E – The injection of normal saline (2.5 µl, i.c.) did not alter the mean frequency or amplitude of penicillin-induced epileptiform activity. Representative ECoGs are presented for 100 min after penicillin administration
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Figure 0001: A – The intracortical injection of penicillin (500 IU/2.5 µl) induced epileptiform activity on ECoG. B – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 70 min after penicillin injection in the 15 min treadmill exercise group (treadmill exercise 15 min/day, for 90 days). C – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 30 min treadmill exercise group (treadmill exercise 30 min/day, for 90 days). D – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 60 min treadmill exercise group (treadmill exercise 60 min/day, for 90 days). E – The injection of normal saline (2.5 µl, i.c.) did not alter the mean frequency or amplitude of penicillin-induced epileptiform activity. Representative ECoGs are presented for 100 min after penicillin administration

Mentions: A single intracortical injection of penicillin (500 IU/2.5 µl dose) induced epileptiform activity approximately 5 min after injection; the activity reached a constant level by 30 min after penicillin administration. Signs of epileptiform activity persisted for approximately 3 h. The means of the spike frequency and amplitude of the epileptiform activity were 49.74 ±16.78 spike/min and 930 ±150 µV, respectively (Figure 1 A).


The effects of treadmill exercise on penicillin-induced epileptiform activity
A – The intracortical injection of penicillin (500 IU/2.5 µl) induced epileptiform activity on ECoG. B – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 70 min after penicillin injection in the 15 min treadmill exercise group (treadmill exercise 15 min/day, for 90 days). C – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 30 min treadmill exercise group (treadmill exercise 30 min/day, for 90 days). D – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 60 min treadmill exercise group (treadmill exercise 60 min/day, for 90 days). E – The injection of normal saline (2.5 µl, i.c.) did not alter the mean frequency or amplitude of penicillin-induced epileptiform activity. Representative ECoGs are presented for 100 min after penicillin administration
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5016582&req=5

Figure 0001: A – The intracortical injection of penicillin (500 IU/2.5 µl) induced epileptiform activity on ECoG. B – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 70 min after penicillin injection in the 15 min treadmill exercise group (treadmill exercise 15 min/day, for 90 days). C – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 30 min treadmill exercise group (treadmill exercise 30 min/day, for 90 days). D – The mean frequency of penicillin-induced epileptiform activity significantly decreased at 90 min after penicillin injection in the 60 min treadmill exercise group (treadmill exercise 60 min/day, for 90 days). E – The injection of normal saline (2.5 µl, i.c.) did not alter the mean frequency or amplitude of penicillin-induced epileptiform activity. Representative ECoGs are presented for 100 min after penicillin administration
Mentions: A single intracortical injection of penicillin (500 IU/2.5 µl dose) induced epileptiform activity approximately 5 min after injection; the activity reached a constant level by 30 min after penicillin administration. Signs of epileptiform activity persisted for approximately 3 h. The means of the spike frequency and amplitude of the epileptiform activity were 49.74 ±16.78 spike/min and 930 ±150 µV, respectively (Figure 1 A).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: The aim of this study was to evaluate the effects of short-, moderate- and long-duration treadmill exercise (15, 30 and 60 min) on the mean frequency and amplitude of penicillin-induced epileptiform activity in rats.

Material and methods: In this study, 32 rats were assigned to 15, 30, and 60 min running exercise groups and the control group, each consisting of 8 rats. According to the specified protocol, the rats were submitted to running exercises at the same hour of each day for 90 days. After the exercise program, the rats were administered (500 IU/2.5 µl) of penicillin into the left cortex by the microinjection method. An electrocorticogram (ECoG) recording was performed for 3 h using a data acquisition system. The frequency and the amplitude of the recordings were analyzed.

Results: Short-duration treadmill exercise (15 min) caused a decrease in the frequency of penicillin-induced epileptiform activity at 70 min after penicillin injection (p < 0.001). The mean frequency of epileptiform activity decreased at 90 min after penicillin injection in the 30 and 60 min treadmill exercise groups (p < 0.01). The mean amplitude of epileptiform activity was not changed in any of the exercise groups compared to the control (p > 0.05).

Conclusions: The results of the present study demonstrate for the first time that short-, moderate- and long-duration treadmill exercises decreased the frequency of penicillin-induced epileptiform activity. These findings may contribute to improving the quality of life in epileptic patients.

No MeSH data available.