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Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: In this study, we aimed to determine the protective effects of hesperidin, a citrus flavonoid, in a model of testicular ischemia/reperfusion injury in rats.

Material and methods: Forty-two pubertal male Wistar-Albino rats were divided into six groups: group 1 – control; group 2 – 50 mg/kg hesperidin (low dose hesperidin) used without torsion (LH group); group 3 – 100 mg/kg hesperidin without torsion (HH group); group 4 – torsion/detorsion group (T/D); group 5 – T/D + 50 mg/kg hesperidin treatment group (T/D + LH); and group 6 – T/D + 100 mg/kg hesperidin treatment group (T/D + HH). Hesperidin was given to the treatment groups 30 min before detorsion. After the fourth hour of reperfusion, orchiectomy was performed on the rats under anesthesia. The tissue samples were examined histologically and biochemically.

Results: In the T/D group testicular malondialdehyde (MDA) levels were increased significantly (p < 0.001) whereas superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels were decreased compared to the control and other groups. However, hesperidin caused the effect of T/D to become closer to normal biochemical values. In addition, the histological examinations showed that T/D caused damage in the testis but hesperidin reduced this effect. The effects of hesperidin were found to be dose dependent. Thus, applying high doses would generate greater therapeutic effects.

Conclusions: In a rat testicular T/D model we observed biochemical and histological damage due to ischemia. However, high and low dose applications of hesperidin were shown to have protective effects against this damage. Therefore, the aforementioned citrus flavonoid may provide positive results in cases of testicular torsion.

No MeSH data available.


Related in: MedlinePlus

T/D Group. Significant coagulative necrosis in germinal cells (Grade IV) (H + E, 400×)
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Figure 0007: T/D Group. Significant coagulative necrosis in germinal cells (Grade IV) (H + E, 400×)

Mentions: Histopathologically, the rats in the control group had essentially normal seminiferous tubule morphology (Figure 1). In the T/D group (group 4), the lesions varied among grades 2 (Figures 2 and 3), 3 and 4. In this group edema, congestion hemorrhage between the seminiferous tubules and necrosis of the germinal cells were the predominant features in the sections (Figures 4–7). When the T/D-HH and T/D groups were compared, in the T/D-HH group, grade 1 was found to be increased (3/7) and grade 3-4 injury was found to be decreased (0/7).


Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
T/D Group. Significant coagulative necrosis in germinal cells (Grade IV) (H + E, 400×)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5016569&req=5

Figure 0007: T/D Group. Significant coagulative necrosis in germinal cells (Grade IV) (H + E, 400×)
Mentions: Histopathologically, the rats in the control group had essentially normal seminiferous tubule morphology (Figure 1). In the T/D group (group 4), the lesions varied among grades 2 (Figures 2 and 3), 3 and 4. In this group edema, congestion hemorrhage between the seminiferous tubules and necrosis of the germinal cells were the predominant features in the sections (Figures 4–7). When the T/D-HH and T/D groups were compared, in the T/D-HH group, grade 1 was found to be increased (3/7) and grade 3-4 injury was found to be decreased (0/7).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: In this study, we aimed to determine the protective effects of hesperidin, a citrus flavonoid, in a model of testicular ischemia/reperfusion injury in rats.

Material and methods: Forty-two pubertal male Wistar-Albino rats were divided into six groups: group 1 – control; group 2 – 50 mg/kg hesperidin (low dose hesperidin) used without torsion (LH group); group 3 – 100 mg/kg hesperidin without torsion (HH group); group 4 – torsion/detorsion group (T/D); group 5 – T/D + 50 mg/kg hesperidin treatment group (T/D + LH); and group 6 – T/D + 100 mg/kg hesperidin treatment group (T/D + HH). Hesperidin was given to the treatment groups 30 min before detorsion. After the fourth hour of reperfusion, orchiectomy was performed on the rats under anesthesia. The tissue samples were examined histologically and biochemically.

Results: In the T/D group testicular malondialdehyde (MDA) levels were increased significantly (p < 0.001) whereas superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels were decreased compared to the control and other groups. However, hesperidin caused the effect of T/D to become closer to normal biochemical values. In addition, the histological examinations showed that T/D caused damage in the testis but hesperidin reduced this effect. The effects of hesperidin were found to be dose dependent. Thus, applying high doses would generate greater therapeutic effects.

Conclusions: In a rat testicular T/D model we observed biochemical and histological damage due to ischemia. However, high and low dose applications of hesperidin were shown to have protective effects against this damage. Therefore, the aforementioned citrus flavonoid may provide positive results in cases of testicular torsion.

No MeSH data available.


Related in: MedlinePlus