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Meroxest improves the prognosis of immunocompetent C57BL/6 mice with allografts of E0771 mouse breast tumor cells

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Recently, we have reported the antitumor properties of a new family of synthetic merosesquiterpenes, among which meroxest is highlighted, since it has high activity and specificity for ER+ breast cancer cells. In this paper, we characterize allografts of ER+ E0771 mouse breast tumor cells in immunocompetent C57BL/6 mice, and also analyze the effect of meroxest on the prognosis of the disease.

Material and methods: Twenty female C57BL/6 mice were injected with 106 E0771 cells. Once the tumors reached the appropriate size, the mice were divided into two groups, one control and another treated orally with 15 mg/kg of meroxest. After 20 days, tumor samples were taken for histopathological study and for determination of the expression of the prognostic markers Ki67 and vascular endothelial growth factor (VEGF) by immunofluorescence.

Results: In sections stained with hematoxylin-eosin, we observed that tumors have a well-defined capsule enclosing E0771 tumor cells. The central area of tumors contains necrotic regions with leukocyte infiltration. Meroxest treatment significantly reduces tumor size (68%, p < 0.05), induces changes in its structure, decreases the degree of leukocyte infiltration, and significantly reduces the expression of Ki67 (33%, p < 0.05) and VEGF (82%, p < 0.05).

Conclusions: Meroxest improves the prognosis of mice since it reduces leukocyte infiltration, and decreases the expression of Ki67 and VEGF markers. Consequently, the merosesquiterpene could become a useful antiangiogenic drug in the treatment of breast cancer. These results encourage us to deepen the study of meroxest, in order to find more evidence that supports the convenience of its evaluation in a clinical study or trial.

No MeSH data available.


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Immunofluorescence analysis of Ki67 and VEGF expression in tumor sections from control and 15 mg/kg meroxest-treated animals. Nuclei were counterstained with DAPI (blue). Bars: 100 µm
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Figure 0004: Immunofluorescence analysis of Ki67 and VEGF expression in tumor sections from control and 15 mg/kg meroxest-treated animals. Nuclei were counterstained with DAPI (blue). Bars: 100 µm

Mentions: We analyzed the expression of the prognostic markers Ki67 and VEGF by immunofluorescence. We observed high expression of Ki67 in tumor cells of control animals. However, animals treated with meroxest experienced a significant decrease in Ki67 expression of over 33%. Similarly, expression of VEGF was significantly reduced by more than 80% in treated animals as compared with controls (Figures 4 and 5).


Meroxest improves the prognosis of immunocompetent C57BL/6 mice with allografts of E0771 mouse breast tumor cells
Immunofluorescence analysis of Ki67 and VEGF expression in tumor sections from control and 15 mg/kg meroxest-treated animals. Nuclei were counterstained with DAPI (blue). Bars: 100 µm
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5016567&req=5

Figure 0004: Immunofluorescence analysis of Ki67 and VEGF expression in tumor sections from control and 15 mg/kg meroxest-treated animals. Nuclei were counterstained with DAPI (blue). Bars: 100 µm
Mentions: We analyzed the expression of the prognostic markers Ki67 and VEGF by immunofluorescence. We observed high expression of Ki67 in tumor cells of control animals. However, animals treated with meroxest experienced a significant decrease in Ki67 expression of over 33%. Similarly, expression of VEGF was significantly reduced by more than 80% in treated animals as compared with controls (Figures 4 and 5).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Recently, we have reported the antitumor properties of a new family of synthetic merosesquiterpenes, among which meroxest is highlighted, since it has high activity and specificity for ER+ breast cancer cells. In this paper, we characterize allografts of ER+ E0771 mouse breast tumor cells in immunocompetent C57BL/6 mice, and also analyze the effect of meroxest on the prognosis of the disease.

Material and methods: Twenty female C57BL/6 mice were injected with 106 E0771 cells. Once the tumors reached the appropriate size, the mice were divided into two groups, one control and another treated orally with 15 mg/kg of meroxest. After 20 days, tumor samples were taken for histopathological study and for determination of the expression of the prognostic markers Ki67 and vascular endothelial growth factor (VEGF) by immunofluorescence.

Results: In sections stained with hematoxylin-eosin, we observed that tumors have a well-defined capsule enclosing E0771 tumor cells. The central area of tumors contains necrotic regions with leukocyte infiltration. Meroxest treatment significantly reduces tumor size (68%, p < 0.05), induces changes in its structure, decreases the degree of leukocyte infiltration, and significantly reduces the expression of Ki67 (33%, p < 0.05) and VEGF (82%, p < 0.05).

Conclusions: Meroxest improves the prognosis of mice since it reduces leukocyte infiltration, and decreases the expression of Ki67 and VEGF markers. Consequently, the merosesquiterpene could become a useful antiangiogenic drug in the treatment of breast cancer. These results encourage us to deepen the study of meroxest, in order to find more evidence that supports the convenience of its evaluation in a clinical study or trial.

No MeSH data available.


Related in: MedlinePlus