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Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal

View Article: PubMed Central - PubMed

ABSTRACT

Taking natural coal as a “seed bank” of bacterial strains able to degrade lignin that is with molecular structure similar to coal components, we isolated 393 and 483 bacterial strains from a meager lean coal sample from Hancheng coalbed and a brown coal sample from Bayannaoer coalbed, respectively, by using different media. Statistical analysis showed that isolates were significantly more site-specific than medium-specific. Of the 876 strains belonging to 27 genera in Actinobacteria, Firmicutes, and Proteobacteria, 612 were positive for lignin degradation function, including 218 strains belonging to 35 species in Hancheng and 394 strains belonging to 19 species in Zhongqi. Among them, the dominant lignin-degrading strains were Thauera (Hancheng), Arthrobacter (Zhongqi) and Rhizobium (both). The genes encoding the laccases- or laccase-like multicopper oxidases, key enzymes in lignin production and degradation, were detected in three genera including Massila for the first time, which was in high expression by real time PCR (qRT-PCR) detection, confirming coal as a good seed bank.

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Abundance of bacteria in Hancheng and Zhongqi samples.(A) Abundance of strains isolated from Hancheng, HM, HW, and HMW. (B) Abundance of strains isolated from Zhongqi, ZQM, ZQW, and ZQMW.
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Figure 3: Abundance of bacteria in Hancheng and Zhongqi samples.(A) Abundance of strains isolated from Hancheng, HM, HW, and HMW. (B) Abundance of strains isolated from Zhongqi, ZQM, ZQW, and ZQMW.

Mentions: Isolates from the genus Thauera dominated those from Hancheng, accounting for 57% of the total; the remaining isolates were from the genera Arthrobacter, Rhizobium, Staphylococcus, Brachybacterium, Micrococcus, Methylobacterium, Tessaracoccus, Brevibacterium, Janibacter, Leucobacter, Devosia, Acinetobacter, Aerococcus, Diaphorobacter, Providencia, Lysinibacillus, Bacillus, Shinella, Proteus, Ancylobacter, and Bosea (Figure 3A). In contrast, Arthrobacter dominated all isolates from Zhongqi (64%), and the remaining isolates were Rhizobium, Staphyloco-ccus, Brachybacterium, Micrococcus, Methylobacterium, Terra-bacter, Brevundimonas, Curtobacterium, Massilia, and Pseudom-onas (Figure 3B). Isolates from Hancheng coal had higher Shannon-Weiner index values, 2.83, at the 16S rRNA gene similarity level, compared to 2.13 of Zhongqi coal (Table 1), suggesting higher bacterial diversity in Hancheng. Strains belonging to Arthrobacter, Rhizobium, Staphylococcus, Brachybacterium, Micrococcus, and Methylobacterium genera were isolated from both Hancheng and Zhongqi, which occupied 32.06 and 97.31% of total isolates, respectively. Strains belonging to Rhizobium could be isolated from all the media from both Hancheng and Zhongqi (Figure 4A). Thauera, Tessaracoccus, Brevibacterium, Janibacter, Leucobacter, Devosia, Acinetobacter, Aerococcus, Diaphorobacter, Providencia, Lysinibacillus, Bacillus, Shinella, Proteus, Ancylobacter, and Bosea were only found in Hancheng (Figure 4B; Supplementary Table S4), and Terrabacter, Brevundimonas, Curtobacterium, Massilia, and Pseudomonas were only found in Zhongqi (Figure 4B; Supplementary Table S4).


Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal
Abundance of bacteria in Hancheng and Zhongqi samples.(A) Abundance of strains isolated from Hancheng, HM, HW, and HMW. (B) Abundance of strains isolated from Zhongqi, ZQM, ZQW, and ZQMW.
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Related In: Results  -  Collection

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Figure 3: Abundance of bacteria in Hancheng and Zhongqi samples.(A) Abundance of strains isolated from Hancheng, HM, HW, and HMW. (B) Abundance of strains isolated from Zhongqi, ZQM, ZQW, and ZQMW.
Mentions: Isolates from the genus Thauera dominated those from Hancheng, accounting for 57% of the total; the remaining isolates were from the genera Arthrobacter, Rhizobium, Staphylococcus, Brachybacterium, Micrococcus, Methylobacterium, Tessaracoccus, Brevibacterium, Janibacter, Leucobacter, Devosia, Acinetobacter, Aerococcus, Diaphorobacter, Providencia, Lysinibacillus, Bacillus, Shinella, Proteus, Ancylobacter, and Bosea (Figure 3A). In contrast, Arthrobacter dominated all isolates from Zhongqi (64%), and the remaining isolates were Rhizobium, Staphyloco-ccus, Brachybacterium, Micrococcus, Methylobacterium, Terra-bacter, Brevundimonas, Curtobacterium, Massilia, and Pseudom-onas (Figure 3B). Isolates from Hancheng coal had higher Shannon-Weiner index values, 2.83, at the 16S rRNA gene similarity level, compared to 2.13 of Zhongqi coal (Table 1), suggesting higher bacterial diversity in Hancheng. Strains belonging to Arthrobacter, Rhizobium, Staphylococcus, Brachybacterium, Micrococcus, and Methylobacterium genera were isolated from both Hancheng and Zhongqi, which occupied 32.06 and 97.31% of total isolates, respectively. Strains belonging to Rhizobium could be isolated from all the media from both Hancheng and Zhongqi (Figure 4A). Thauera, Tessaracoccus, Brevibacterium, Janibacter, Leucobacter, Devosia, Acinetobacter, Aerococcus, Diaphorobacter, Providencia, Lysinibacillus, Bacillus, Shinella, Proteus, Ancylobacter, and Bosea were only found in Hancheng (Figure 4B; Supplementary Table S4), and Terrabacter, Brevundimonas, Curtobacterium, Massilia, and Pseudomonas were only found in Zhongqi (Figure 4B; Supplementary Table S4).

View Article: PubMed Central - PubMed

ABSTRACT

Taking natural coal as a “seed bank” of bacterial strains able to degrade lignin that is with molecular structure similar to coal components, we isolated 393 and 483 bacterial strains from a meager lean coal sample from Hancheng coalbed and a brown coal sample from Bayannaoer coalbed, respectively, by using different media. Statistical analysis showed that isolates were significantly more site-specific than medium-specific. Of the 876 strains belonging to 27 genera in Actinobacteria, Firmicutes, and Proteobacteria, 612 were positive for lignin degradation function, including 218 strains belonging to 35 species in Hancheng and 394 strains belonging to 19 species in Zhongqi. Among them, the dominant lignin-degrading strains were Thauera (Hancheng), Arthrobacter (Zhongqi) and Rhizobium (both). The genes encoding the laccases- or laccase-like multicopper oxidases, key enzymes in lignin production and degradation, were detected in three genera including Massila for the first time, which was in high expression by real time PCR (qRT-PCR) detection, confirming coal as a good seed bank.

No MeSH data available.