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Effects of Lizhong Tang on gastrointestinal motility in mice

View Article: PubMed Central - PubMed

ABSTRACT

Aim: To investigate the effects of Lizhong Tang, a traditional Chinese medicine formula, on gastrointestinal motility in mice.

Methods: The in vivo effects of Lizhong Tang on GI motility were investigated by measuring the intestinal transit rates (ITRs) and gastric emptying (GE) values in normal mice and in mice with experimentally induced GI motility dysfunction (GMD).

Results: In normal ICR mice, the ITR and GE values were significantly and dose-dependently increased by Lizhong Tang (ITR values: 54.4% ± 1.9% vs 65.2% ± 1.8%, P < 0.01 with 0.1 g/kg Lizhong Tang and 54.4% ± 1.9% vs 83.8% ± 1.9%, P < 0.01 with 1 g/kg Lizhong Tang; GE values: 60.7% ± 1.9% vs 66.8% ± 2.1%, P < 0.05 with 0.1 g/kg Lizhong Tang and 60.7% ± 1.9% vs 72.5% ± 1.7%, P < 0.01 with 1 g/kg Lizhong Tang). The ITRs of the GMD mice were significantly reduced compared with those of the normal mice, which were significantly and dose-dependently reversed by Lizhong Tang. Additionally, in loperamide- and cisplatin-induced models of GE delay, Lizhong Tang administration reversed the GE deficits.

Conclusion: These results suggest that Lizhong Tang may be a novel candidate for development as a prokinetic treatment for the GI tract.

No MeSH data available.


Lizhong Tang extract ameliorated cisplatin-induced delays in gastric emptying. After a 24 h fast, the animals (n = 6/each group) were orally administered the indicated dosages of the Lizhong Tang extract, 5 mg/kg of a 5-HT4 receptor agonist (mosapride), 5 mg/kg of a dopamine receptor antagonist (domperidone), or distilled water (DW; control). The Lizhong Tang pretreatment prevented the cisplatin-induced gastric emptying (GE) delay. The GE percentages were calculated as described in the Materials and Methods. The bars represent mean values ± SE. bP < 0.01; significantly different from the normal controls. Mosa: Mosapride; Dom: Domperidone; i.p: Intraperitoneally.
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Figure 7: Lizhong Tang extract ameliorated cisplatin-induced delays in gastric emptying. After a 24 h fast, the animals (n = 6/each group) were orally administered the indicated dosages of the Lizhong Tang extract, 5 mg/kg of a 5-HT4 receptor agonist (mosapride), 5 mg/kg of a dopamine receptor antagonist (domperidone), or distilled water (DW; control). The Lizhong Tang pretreatment prevented the cisplatin-induced gastric emptying (GE) delay. The GE percentages were calculated as described in the Materials and Methods. The bars represent mean values ± SE. bP < 0.01; significantly different from the normal controls. Mosa: Mosapride; Dom: Domperidone; i.p: Intraperitoneally.

Mentions: In normal mice, the groups treated with the Lizhong Tang extract (0.01, 0.1 and 1 g/kg) showed significantly enhanced GE (%) values compared to that of the normal group [the GE values with 0.01, 0.1 and 1 g/kg of the Lizhong Tang extract were 61.7% ± 1.6%, 66.8% ± 2.1% (P < 0.05) and 72.5% ± 1.7% (P < 0.01), respectively; Figure 5]. Its effects were dose-dependent in the dosage range from 0.01 g/kg to 1 g/kg, and 1 g/kg of the Lizhong Tang extract displayed effects similar to those of 5 mg/kg mosapride [74.4% ± 3.3% (P < 0.01)] and 5 mg/kg domperidone [72.9% ± 1.9% (P < 0.01)] (Figure 5). Next, we examined loperamide-induced and cisplatin-induced models of GE delay to determine whether the Lizhong Tang extract could increase GE in abnormally depressed GE models. In the loperamide-induced model of GE delay, the GE value was lower than normal [40.9% ± 1.6% (P < 0.01); Figure 6], and this decrease was recovered by treatment with the Lizhong Tang extract at doses from 0.01 to 1 g/kg [the GE values for the Lizhong Tang extract at 0.01, 0.1 and 1 g/kg were 41.8% ± 2.2%, 47.8% ± 1.2% (P < 0.01) and 59.4% ± 1.5% (P < 0.01), respectively; Figure 6]. The maximal effect was obtained at 1 g/kg, and at this dose, the effect of the Lizhong Tang extract was comparable to that of 5 mg/kg mosapride [61.4% ± 2.3% (P < 0.01)] or 5 mg/kg domperidone [61.5% ± 1.7% (P < 0.01)] (Figure 6). In addition, in the cisplatin-induced model of GE delay, the decreased GE was recovered by treatment with the Lizhong Tang extract (0.01, 0.1 and 1 g/kg) [GE values of the Lizhong Tang extract at 0.01, 0.1 and 1 g/kg were 31.7% ± 1.3%, 43.1% ± 2.1% (P < 0.01) and 60.8% ± 1.7% (P < 0.01), respectively; Figure 7]. The maximal effect was obtained at 1 g/kg, and at this level, the effect of the Lizhong Tang extract was comparable to that of 5 mg/kg mosapride [65.6% ± 1.4% (P < 0.01)] or 5 mg/kg domperidone [63.7% ± 1.2% (P < 0.01)] (Figure 7).


Effects of Lizhong Tang on gastrointestinal motility in mice
Lizhong Tang extract ameliorated cisplatin-induced delays in gastric emptying. After a 24 h fast, the animals (n = 6/each group) were orally administered the indicated dosages of the Lizhong Tang extract, 5 mg/kg of a 5-HT4 receptor agonist (mosapride), 5 mg/kg of a dopamine receptor antagonist (domperidone), or distilled water (DW; control). The Lizhong Tang pretreatment prevented the cisplatin-induced gastric emptying (GE) delay. The GE percentages were calculated as described in the Materials and Methods. The bars represent mean values ± SE. bP < 0.01; significantly different from the normal controls. Mosa: Mosapride; Dom: Domperidone; i.p: Intraperitoneally.
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Figure 7: Lizhong Tang extract ameliorated cisplatin-induced delays in gastric emptying. After a 24 h fast, the animals (n = 6/each group) were orally administered the indicated dosages of the Lizhong Tang extract, 5 mg/kg of a 5-HT4 receptor agonist (mosapride), 5 mg/kg of a dopamine receptor antagonist (domperidone), or distilled water (DW; control). The Lizhong Tang pretreatment prevented the cisplatin-induced gastric emptying (GE) delay. The GE percentages were calculated as described in the Materials and Methods. The bars represent mean values ± SE. bP < 0.01; significantly different from the normal controls. Mosa: Mosapride; Dom: Domperidone; i.p: Intraperitoneally.
Mentions: In normal mice, the groups treated with the Lizhong Tang extract (0.01, 0.1 and 1 g/kg) showed significantly enhanced GE (%) values compared to that of the normal group [the GE values with 0.01, 0.1 and 1 g/kg of the Lizhong Tang extract were 61.7% ± 1.6%, 66.8% ± 2.1% (P < 0.05) and 72.5% ± 1.7% (P < 0.01), respectively; Figure 5]. Its effects were dose-dependent in the dosage range from 0.01 g/kg to 1 g/kg, and 1 g/kg of the Lizhong Tang extract displayed effects similar to those of 5 mg/kg mosapride [74.4% ± 3.3% (P < 0.01)] and 5 mg/kg domperidone [72.9% ± 1.9% (P < 0.01)] (Figure 5). Next, we examined loperamide-induced and cisplatin-induced models of GE delay to determine whether the Lizhong Tang extract could increase GE in abnormally depressed GE models. In the loperamide-induced model of GE delay, the GE value was lower than normal [40.9% ± 1.6% (P < 0.01); Figure 6], and this decrease was recovered by treatment with the Lizhong Tang extract at doses from 0.01 to 1 g/kg [the GE values for the Lizhong Tang extract at 0.01, 0.1 and 1 g/kg were 41.8% ± 2.2%, 47.8% ± 1.2% (P < 0.01) and 59.4% ± 1.5% (P < 0.01), respectively; Figure 6]. The maximal effect was obtained at 1 g/kg, and at this dose, the effect of the Lizhong Tang extract was comparable to that of 5 mg/kg mosapride [61.4% ± 2.3% (P < 0.01)] or 5 mg/kg domperidone [61.5% ± 1.7% (P < 0.01)] (Figure 6). In addition, in the cisplatin-induced model of GE delay, the decreased GE was recovered by treatment with the Lizhong Tang extract (0.01, 0.1 and 1 g/kg) [GE values of the Lizhong Tang extract at 0.01, 0.1 and 1 g/kg were 31.7% ± 1.3%, 43.1% ± 2.1% (P < 0.01) and 60.8% ± 1.7% (P < 0.01), respectively; Figure 7]. The maximal effect was obtained at 1 g/kg, and at this level, the effect of the Lizhong Tang extract was comparable to that of 5 mg/kg mosapride [65.6% ± 1.4% (P < 0.01)] or 5 mg/kg domperidone [63.7% ± 1.2% (P < 0.01)] (Figure 7).

View Article: PubMed Central - PubMed

ABSTRACT

Aim: To investigate the effects of Lizhong Tang, a traditional Chinese medicine formula, on gastrointestinal motility in mice.

Methods: The in vivo effects of Lizhong Tang on GI motility were investigated by measuring the intestinal transit rates (ITRs) and gastric emptying (GE) values in normal mice and in mice with experimentally induced GI motility dysfunction (GMD).

Results: In normal ICR mice, the ITR and GE values were significantly and dose-dependently increased by Lizhong Tang (ITR values: 54.4% &plusmn; 1.9% vs 65.2% &plusmn; 1.8%, P &lt; 0.01 with 0.1 g/kg Lizhong Tang and 54.4% &plusmn; 1.9% vs 83.8% &plusmn; 1.9%, P &lt; 0.01 with 1 g/kg Lizhong Tang; GE values: 60.7% &plusmn; 1.9% vs 66.8% &plusmn; 2.1%, P &lt; 0.05 with 0.1 g/kg Lizhong Tang and 60.7% &plusmn; 1.9% vs 72.5% &plusmn; 1.7%, P &lt; 0.01 with 1 g/kg Lizhong Tang). The ITRs of the GMD mice were significantly reduced compared with those of the normal mice, which were significantly and dose-dependently reversed by Lizhong Tang. Additionally, in loperamide- and cisplatin-induced models of GE delay, Lizhong Tang administration reversed the GE deficits.

Conclusion: These results suggest that Lizhong Tang may be a novel candidate for development as a prokinetic treatment for the GI tract.

No MeSH data available.