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Plasma Macrophage Migration Inhibitor Factor Is Elevated in Response to Myocardial Ischemia

View Article: PubMed Central - PubMed

ABSTRACT

Background: Macrophage migration inhibitory factor (MIF) is a key regulator of inflammatory responses, including in the heart. Plasma MIF is elevated early in the course of acute myocardial infarction. In this study, we hypothesized that plasma MIF may also be increased in acute myocardial ischemia.

Methods and results: Patients undergoing cardiac stress test (stress nuclear myocardial perfusion scan or stress echocardiography) were recruited. Twenty‐two patients had a stress test indicative of myocardial ischemia and were compared with 62 patients who had a negative stress test. Plasma MIF was measured by ELISA before and after the stress test. MIF was also measured in patients with peripheral arterial occlusive disease before and after exercise causing claudication. Gene and protein expression of MIF was measured in mouse cardiac and skeletal muscle tissue by real‐time polymerase chain reaction and western blot, respectively. Plasma MIF was elevated at 5 and 15 minutes after stress (relative to before stress) in patients with a positive test, compared with those with a negative test. In contrast, high‐sensitivity troponin T and C‐reactive protein were not altered after stress in either group. MIF was not altered after exercise in PAOD patients, despite the occurrence of claudication, suggesting that plasma MIF is not a marker for skeletal muscle ischemia. This may be explained by a lower gene and protein expression of MIF in skeletal muscle than the heart.

Conclusions: Our results suggest that plasma MIF is an early marker for acute myocardial ischemia.

No MeSH data available.


Related in: MedlinePlus

Plasma MIF was measured in peripheral venous blood before and in distal left anterior descending artery before and immediately after balloon angioplasty (A, n=9). Plasma MIF was measured before and 5 and 15 minutes after exercise in patients with peripheral arterial occlusive disease (PAOD; B, n=10). Data are expressed as mean±SEM. MIF indicates macrophage migration inhibitory factor.
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jah31593-fig-0003: Plasma MIF was measured in peripheral venous blood before and in distal left anterior descending artery before and immediately after balloon angioplasty (A, n=9). Plasma MIF was measured before and 5 and 15 minutes after exercise in patients with peripheral arterial occlusive disease (PAOD; B, n=10). Data are expressed as mean±SEM. MIF indicates macrophage migration inhibitory factor.

Mentions: Basic characteristics of patients are presented in Table 2. Coronary plasma MIF level did not change after balloon angioplasty (P>0.05; Figure 3A).


Plasma Macrophage Migration Inhibitor Factor Is Elevated in Response to Myocardial Ischemia
Plasma MIF was measured in peripheral venous blood before and in distal left anterior descending artery before and immediately after balloon angioplasty (A, n=9). Plasma MIF was measured before and 5 and 15 minutes after exercise in patients with peripheral arterial occlusive disease (PAOD; B, n=10). Data are expressed as mean±SEM. MIF indicates macrophage migration inhibitory factor.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5015363&req=5

jah31593-fig-0003: Plasma MIF was measured in peripheral venous blood before and in distal left anterior descending artery before and immediately after balloon angioplasty (A, n=9). Plasma MIF was measured before and 5 and 15 minutes after exercise in patients with peripheral arterial occlusive disease (PAOD; B, n=10). Data are expressed as mean±SEM. MIF indicates macrophage migration inhibitory factor.
Mentions: Basic characteristics of patients are presented in Table 2. Coronary plasma MIF level did not change after balloon angioplasty (P>0.05; Figure 3A).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Macrophage migration inhibitory factor (MIF) is a key regulator of inflammatory responses, including in the heart. Plasma MIF is elevated early in the course of acute myocardial infarction. In this study, we hypothesized that plasma MIF may also be increased in acute myocardial ischemia.

Methods and results: Patients undergoing cardiac stress test (stress nuclear myocardial perfusion scan or stress echocardiography) were recruited. Twenty‐two patients had a stress test indicative of myocardial ischemia and were compared with 62 patients who had a negative stress test. Plasma MIF was measured by ELISA before and after the stress test. MIF was also measured in patients with peripheral arterial occlusive disease before and after exercise causing claudication. Gene and protein expression of MIF was measured in mouse cardiac and skeletal muscle tissue by real‐time polymerase chain reaction and western blot, respectively. Plasma MIF was elevated at 5 and 15 minutes after stress (relative to before stress) in patients with a positive test, compared with those with a negative test. In contrast, high‐sensitivity troponin T and C‐reactive protein were not altered after stress in either group. MIF was not altered after exercise in PAOD patients, despite the occurrence of claudication, suggesting that plasma MIF is not a marker for skeletal muscle ischemia. This may be explained by a lower gene and protein expression of MIF in skeletal muscle than the heart.

Conclusions: Our results suggest that plasma MIF is an early marker for acute myocardial ischemia.

No MeSH data available.


Related in: MedlinePlus