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Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon – bone healing in a rabbit model of anterior cruciate ligament reconstruction

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ABSTRACT

Background: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon–bone healing in a rabbit model.

Methods: In the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon–bone healing by histology, radiology, and biomechanical testing.

Results: The in vitro study revealed that collagen I, osteocalcin, and osteopontin expression was higher in BMSCs co-cultured with PRP for 14 days. The in vivo study revealed a more mature tendon–bone interface using light microscopy, a more newly formed bone at the bone tunnel walls detected by micro-computed tomography, and a significantly higher failure load as assessed by biomechanical testing in the BMSC + PRP group than in the control and PRP groups.

Conclusions: These results indicate that the combination of PRP and BMSCs promotes tendon–bone healing and has potential for clinical use.

Electronic supplementary material: The online version of this article (doi:10.1186/s13018-016-0433-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Histological observations of the tendon–bone interface in the control (a, d), PRP (b, e), and BMSC + PRP groups (c, f) by haematoxylin and eosin (H&E) (a–c) and Russell–Movat pentachrome staining (d–f). Magnification, ×100; scale bar, 100 μm. t tendon graft; b bone; if interface
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Fig6: Histological observations of the tendon–bone interface in the control (a, d), PRP (b, e), and BMSC + PRP groups (c, f) by haematoxylin and eosin (H&E) (a–c) and Russell–Movat pentachrome staining (d–f). Magnification, ×100; scale bar, 100 μm. t tendon graft; b bone; if interface

Mentions: Organised fibrous tissue and some new bone containing chondrocytes were observed at the tendon–bone interface in the control group 8 weeks postoperatively (Fig. 6a, d). Aligned connective tissue, newly formed woven bone, and cartilage were observed at the tendon–bone interface in the PRP group (Fig. 6b, e). A more mature interface with aligned chondrocytes was observed in the BMSC + PRP group, but the fibrous connective tissue at the tendon–bone interface was unclear. A more aligned and layered cartilage zone was observed, which incorporated adjacent bone and tendon (Fig. 6c, f).Fig. 6


Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon – bone healing in a rabbit model of anterior cruciate ligament reconstruction
Histological observations of the tendon–bone interface in the control (a, d), PRP (b, e), and BMSC + PRP groups (c, f) by haematoxylin and eosin (H&E) (a–c) and Russell–Movat pentachrome staining (d–f). Magnification, ×100; scale bar, 100 μm. t tendon graft; b bone; if interface
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5015347&req=5

Fig6: Histological observations of the tendon–bone interface in the control (a, d), PRP (b, e), and BMSC + PRP groups (c, f) by haematoxylin and eosin (H&E) (a–c) and Russell–Movat pentachrome staining (d–f). Magnification, ×100; scale bar, 100 μm. t tendon graft; b bone; if interface
Mentions: Organised fibrous tissue and some new bone containing chondrocytes were observed at the tendon–bone interface in the control group 8 weeks postoperatively (Fig. 6a, d). Aligned connective tissue, newly formed woven bone, and cartilage were observed at the tendon–bone interface in the PRP group (Fig. 6b, e). A more mature interface with aligned chondrocytes was observed in the BMSC + PRP group, but the fibrous connective tissue at the tendon–bone interface was unclear. A more aligned and layered cartilage zone was observed, which incorporated adjacent bone and tendon (Fig. 6c, f).Fig. 6

View Article: PubMed Central - PubMed

ABSTRACT

Background: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon–bone healing in a rabbit model.

Methods: In the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon–bone healing by histology, radiology, and biomechanical testing.

Results: The in vitro study revealed that collagen I, osteocalcin, and osteopontin expression was higher in BMSCs co-cultured with PRP for 14 days. The in vivo study revealed a more mature tendon–bone interface using light microscopy, a more newly formed bone at the bone tunnel walls detected by micro-computed tomography, and a significantly higher failure load as assessed by biomechanical testing in the BMSC + PRP group than in the control and PRP groups.

Conclusions: These results indicate that the combination of PRP and BMSCs promotes tendon–bone healing and has potential for clinical use.

Electronic supplementary material: The online version of this article (doi:10.1186/s13018-016-0433-7) contains supplementary material, which is available to authorized users.

No MeSH data available.