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Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon – bone healing in a rabbit model of anterior cruciate ligament reconstruction

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ABSTRACT

Background: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon–bone healing in a rabbit model.

Methods: In the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon–bone healing by histology, radiology, and biomechanical testing.

Results: The in vitro study revealed that collagen I, osteocalcin, and osteopontin expression was higher in BMSCs co-cultured with PRP for 14 days. The in vivo study revealed a more mature tendon–bone interface using light microscopy, a more newly formed bone at the bone tunnel walls detected by micro-computed tomography, and a significantly higher failure load as assessed by biomechanical testing in the BMSC + PRP group than in the control and PRP groups.

Conclusions: These results indicate that the combination of PRP and BMSCs promotes tendon–bone healing and has potential for clinical use.

Electronic supplementary material: The online version of this article (doi:10.1186/s13018-016-0433-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Alizarin red (a), oil red O (b), and alcian blue staining (c) to detect the differentiating ability of bone marrow mesenchymal stem cells (BMSCs) relative to the bone, fat, and cartilage, respectively. Scale bar, 50 μm
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Fig4: Alizarin red (a), oil red O (b), and alcian blue staining (c) to detect the differentiating ability of bone marrow mesenchymal stem cells (BMSCs) relative to the bone, fat, and cartilage, respectively. Scale bar, 50 μm

Mentions: A total of 69.2 and 99.7 % of the passage 3 cultured cells expressed CD44 and CD90, respectively, whereas only 4.82 % expressed CD45 and were identified as BMSCs (Fig. 3). Following 3 weeks of culture in osteogenesis induction medium followed by alizarin red staining, obvious mineralised nodules were observed under the microscope (Fig. 4a). Oil red O staining revealed many lipid droplets (Fig. 4b), and the BMSC cytoplasm was stained green by alcian blue after 3 weeks of chondrogenic induction (Fig. 4c).Fig. 3


Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon – bone healing in a rabbit model of anterior cruciate ligament reconstruction
Alizarin red (a), oil red O (b), and alcian blue staining (c) to detect the differentiating ability of bone marrow mesenchymal stem cells (BMSCs) relative to the bone, fat, and cartilage, respectively. Scale bar, 50 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5015347&req=5

Fig4: Alizarin red (a), oil red O (b), and alcian blue staining (c) to detect the differentiating ability of bone marrow mesenchymal stem cells (BMSCs) relative to the bone, fat, and cartilage, respectively. Scale bar, 50 μm
Mentions: A total of 69.2 and 99.7 % of the passage 3 cultured cells expressed CD44 and CD90, respectively, whereas only 4.82 % expressed CD45 and were identified as BMSCs (Fig. 3). Following 3 weeks of culture in osteogenesis induction medium followed by alizarin red staining, obvious mineralised nodules were observed under the microscope (Fig. 4a). Oil red O staining revealed many lipid droplets (Fig. 4b), and the BMSC cytoplasm was stained green by alcian blue after 3 weeks of chondrogenic induction (Fig. 4c).Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Background: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon–bone healing in a rabbit model.

Methods: In the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon–bone healing by histology, radiology, and biomechanical testing.

Results: The in vitro study revealed that collagen I, osteocalcin, and osteopontin expression was higher in BMSCs co-cultured with PRP for 14 days. The in vivo study revealed a more mature tendon–bone interface using light microscopy, a more newly formed bone at the bone tunnel walls detected by micro-computed tomography, and a significantly higher failure load as assessed by biomechanical testing in the BMSC + PRP group than in the control and PRP groups.

Conclusions: These results indicate that the combination of PRP and BMSCs promotes tendon–bone healing and has potential for clinical use.

Electronic supplementary material: The online version of this article (doi:10.1186/s13018-016-0433-7) contains supplementary material, which is available to authorized users.

No MeSH data available.