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Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus

Cell proliferation. Cell proliferation was measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment followed by proliferation periods of 24, 48, 72 and 96 h in MDA-MB-231 (a), MCF7 (b) and MCF10A (c) cells. Data are represented as the mean (±SEM) with respect to the controls, which were set as 100 %, for three independent assays carried out in triplicate. *, † and ∆ denote statistically significant differences relative to the control at p < 0.05 for MDA-MB-231, MCF7 or MCF10A, respectively
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Fig5: Cell proliferation. Cell proliferation was measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment followed by proliferation periods of 24, 48, 72 and 96 h in MDA-MB-231 (a), MCF7 (b) and MCF10A (c) cells. Data are represented as the mean (±SEM) with respect to the controls, which were set as 100 %, for three independent assays carried out in triplicate. *, † and ∆ denote statistically significant differences relative to the control at p < 0.05 for MDA-MB-231, MCF7 or MCF10A, respectively

Mentions: Proliferation of MDA-MB-231 (Fig. 5a), MCF7 (Fig. 5b) and MCF10A cells (Fig. 5c) was determined after treatment with PINO for 24 h followed by incubation with fresh medium. Measurements were performed at 24, 48, 72 and 96 h following treatment removal. At 0.001, 0.01 and 0.1 μM, cell survival was inhibited in MDA-MB-231 and MCF7 cells. Surprisingly, at 0.001 μM, proliferation was reduced in tumour cells, but not in mammary epithelial cells, in a statistically significant manner. Strong proliferation was observed in MCF10A cells treated with up to 100 μM PINO, whereas neither MDA-MB-231 nor MCF7 showed this effect.Fig. 5


Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses
Cell proliferation. Cell proliferation was measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment followed by proliferation periods of 24, 48, 72 and 96 h in MDA-MB-231 (a), MCF7 (b) and MCF10A (c) cells. Data are represented as the mean (±SEM) with respect to the controls, which were set as 100 %, for three independent assays carried out in triplicate. *, † and ∆ denote statistically significant differences relative to the control at p < 0.05 for MDA-MB-231, MCF7 or MCF10A, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5015324&req=5

Fig5: Cell proliferation. Cell proliferation was measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment followed by proliferation periods of 24, 48, 72 and 96 h in MDA-MB-231 (a), MCF7 (b) and MCF10A (c) cells. Data are represented as the mean (±SEM) with respect to the controls, which were set as 100 %, for three independent assays carried out in triplicate. *, † and ∆ denote statistically significant differences relative to the control at p < 0.05 for MDA-MB-231, MCF7 or MCF10A, respectively
Mentions: Proliferation of MDA-MB-231 (Fig. 5a), MCF7 (Fig. 5b) and MCF10A cells (Fig. 5c) was determined after treatment with PINO for 24 h followed by incubation with fresh medium. Measurements were performed at 24, 48, 72 and 96 h following treatment removal. At 0.001, 0.01 and 0.1 μM, cell survival was inhibited in MDA-MB-231 and MCF7 cells. Surprisingly, at 0.001 μM, proliferation was reduced in tumour cells, but not in mammary epithelial cells, in a statistically significant manner. Strong proliferation was observed in MCF10A cells treated with up to 100 μM PINO, whereas neither MDA-MB-231 nor MCF7 showed this effect.Fig. 5

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus