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Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity assay. Cell survival measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment on MDA-MB-231, MCF7 and MCF10A cells. Data are represented as the treatment average (±SEM) with respect to the control, which was considered as 100 %, for three independent assays carried out in triplicate. * MDA-MB-231, † MCF7 and ∆ MCF10A indicate statistically significant differences at p < 0.05
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Fig4: Cytotoxicity assay. Cell survival measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment on MDA-MB-231, MCF7 and MCF10A cells. Data are represented as the treatment average (±SEM) with respect to the control, which was considered as 100 %, for three independent assays carried out in triplicate. * MDA-MB-231, † MCF7 and ∆ MCF10A indicate statistically significant differences at p < 0.05

Mentions: To assess the potential cytotoxic effects of PINO, MDA-MB-231, MCF7 and MCF10A cells were treated with concentrations of PINO ranging from 0.001 to 100 μM for 24 h. Surprisingly, PINO treatment was shown to promote a widespread cytotoxic effect at low concentrations in MCF7 cells and at all of the concentrations tested in MDA-MB-231 cells, although statistically significant changes were only observed from 0.001 to 1 μM (Fig. 4). Importantly, the percentage of non-tumorigenic human mammary epithelial cells death following treatment with 0.001 μM PINO was much lower (10 %) than in breast cancer cells (29 % for MDA-MB-231 and 20 % for MCF7 cells). In addition, a 10 μM PINO dose was shown to inhibit proliferation in MCF7 cells but did not induce significant cytotoxicity in MCF10A. Interestingly, a statistically significant cytotoxic effect was observed following 0.01 μM PINO treatment in both types of human breast tumour cells tested, but not in human mammary epithelial cells.Fig. 4


Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses
Cytotoxicity assay. Cell survival measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment on MDA-MB-231, MCF7 and MCF10A cells. Data are represented as the treatment average (±SEM) with respect to the control, which was considered as 100 %, for three independent assays carried out in triplicate. * MDA-MB-231, † MCF7 and ∆ MCF10A indicate statistically significant differences at p < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5015324&req=5

Fig4: Cytotoxicity assay. Cell survival measured by CellTiter-Blue® after 24 h of (+)-pinoresinol treatment on MDA-MB-231, MCF7 and MCF10A cells. Data are represented as the treatment average (±SEM) with respect to the control, which was considered as 100 %, for three independent assays carried out in triplicate. * MDA-MB-231, † MCF7 and ∆ MCF10A indicate statistically significant differences at p < 0.05
Mentions: To assess the potential cytotoxic effects of PINO, MDA-MB-231, MCF7 and MCF10A cells were treated with concentrations of PINO ranging from 0.001 to 100 μM for 24 h. Surprisingly, PINO treatment was shown to promote a widespread cytotoxic effect at low concentrations in MCF7 cells and at all of the concentrations tested in MDA-MB-231 cells, although statistically significant changes were only observed from 0.001 to 1 μM (Fig. 4). Importantly, the percentage of non-tumorigenic human mammary epithelial cells death following treatment with 0.001 μM PINO was much lower (10 %) than in breast cancer cells (29 % for MDA-MB-231 and 20 % for MCF7 cells). In addition, a 10 μM PINO dose was shown to inhibit proliferation in MCF7 cells but did not induce significant cytotoxicity in MCF10A. Interestingly, a statistically significant cytotoxic effect was observed following 0.01 μM PINO treatment in both types of human breast tumour cells tested, but not in human mammary epithelial cells.Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus