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Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


ABTS radical scavenging assay. ABTS●+ radical scavenging activity of (+)-pinoresinol a or TroloxTMb at concentrations between 0.01and 1000 μM and 50 and 800 μM, respectively. Data are expressed as the absorbance of ABTS●+, which was read at 734 nm for 2 h. Measurements were performed in triplicate in three independent experiments
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Fig2: ABTS radical scavenging assay. ABTS●+ radical scavenging activity of (+)-pinoresinol a or TroloxTMb at concentrations between 0.01and 1000 μM and 50 and 800 μM, respectively. Data are expressed as the absorbance of ABTS●+, which was read at 734 nm for 2 h. Measurements were performed in triplicate in three independent experiments

Mentions: The radical cation ABTS●+ was diminished by pinoresinol (PINO) above 10 μM (Fig. 2), and the studied range was from 0.00001 to 1000 μM. Concentrations lower than 10 μM did not shown antioxidant capacity (data not shown). The antioxidant effects of PINO were higher than the antioxidant standard, as the 50 % of Radical Scavenging Activity (RSA) occurred at 380 μM for Trolox™ and at 274 μM for PINO.Fig. 2


Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses
ABTS radical scavenging assay. ABTS●+ radical scavenging activity of (+)-pinoresinol a or TroloxTMb at concentrations between 0.01and 1000 μM and 50 and 800 μM, respectively. Data are expressed as the absorbance of ABTS●+, which was read at 734 nm for 2 h. Measurements were performed in triplicate in three independent experiments
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5015324&req=5

Fig2: ABTS radical scavenging assay. ABTS●+ radical scavenging activity of (+)-pinoresinol a or TroloxTMb at concentrations between 0.01and 1000 μM and 50 and 800 μM, respectively. Data are expressed as the absorbance of ABTS●+, which was read at 734 nm for 2 h. Measurements were performed in triplicate in three independent experiments
Mentions: The radical cation ABTS●+ was diminished by pinoresinol (PINO) above 10 μM (Fig. 2), and the studied range was from 0.00001 to 1000 μM. Concentrations lower than 10 μM did not shown antioxidant capacity (data not shown). The antioxidant effects of PINO were higher than the antioxidant standard, as the 50 % of Radical Scavenging Activity (RSA) occurred at 380 μM for Trolox™ and at 274 μM for PINO.Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.