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Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.


Chemical structure of (+)-pinoresinol
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Fig1: Chemical structure of (+)-pinoresinol

Mentions: One of the most abundant phenolic compounds in VOOs, behind tyrosol and hydroxytyrosol, is (+)-pinoresinol (PINO) [8] (Fig. 1). Its presence in VOOs depend on the variety of the cultivar, in amounts of 0.07 ± 0.003 mg/kg in Arbequina variety, about 0.90 ± 0.78 in Picual variety [9]. Brenes et al. [10] reported that Spanish olive oil contains a range of 20 to 45 mg/kg PINO. Several health properties have been attributed to PINO, including antifungal [11], anti-inflammatory [12, 13], hypoglycaemic [14] or chemopreventive biological activities [15, 16].Fig. 1


Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses
Chemical structure of (+)-pinoresinol
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5015324&req=5

Fig1: Chemical structure of (+)-pinoresinol
Mentions: One of the most abundant phenolic compounds in VOOs, behind tyrosol and hydroxytyrosol, is (+)-pinoresinol (PINO) [8] (Fig. 1). Its presence in VOOs depend on the variety of the cultivar, in amounts of 0.07 ± 0.003 mg/kg in Arbequina variety, about 0.90 ± 0.78 in Picual variety [9]. Brenes et al. [10] reported that Spanish olive oil contains a range of 20 to 45 mg/kg PINO. Several health properties have been attributed to PINO, including antifungal [11], anti-inflammatory [12, 13], hypoglycaemic [14] or chemopreventive biological activities [15, 16].Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

Background: Consumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.

Methods: To address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.

Results: Pinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.

Conclusions: Overall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.

Electronic supplementary material: The online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

No MeSH data available.