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Cytomegalovirus ‐ Productive Infection Is Associated With Acute Coronary Syndrome

View Article: PubMed Central - PubMed

ABSTRACT

Background: Although an association between human herpesvirus (HHV) infection and atherosclerosis has been suggested, the data supporting such an association are controversial and, in most cases, are based on serological evidence or on the presence of cell‐associated HHV DNA, which do not report about actual viral replication. We quantified the DNA of all 8 types of HHVs in plasma, in which their presence is evidence of viral replication.

Methods and results: Using quantitative real‐time polymerase chain reaction, we evaluated the presence of HHV DNA in blood samples obtained at the time of hospitalization from 71 patients with acute coronary syndrome, 26 patients with stable coronary artery disease, and 53 healthy volunteers and in atherosclerotic plaques of 22 patients with peripheral artery disease who underwent endarterectomy. HHV‐5 (cytomegalovirus [CMV]) was the only HHV with a level that was higher in acute coronary syndrome patients than in the control group and that correlated with the level of high‐sensitivity C‐reactive protein. The numbers of effector memory T cells positively correlated with the numbers of CMV genome copies in carotid arteries plaques, whereas the numbers of central memory T cells negatively correlated with CMV copy numbers.

Conclusions: Of all HHV levels, only CMV was higher in patients with stable coronary artery disease and acute coronary syndrome than in the healthy group, and its load correlated with the level of high‐sensitivity C‐reactive protein. The level of CMV in atherosclerotic plaques correlated with the state of immunoactivation of lymphocytes in plaques, suggesting that the reactivation of CMV may contribute to the immune activation associated with the progression of atherosclerosis.

No MeSH data available.


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Correlation coefficient with 95% CI according to Spearman rank test between CMV DNA load and fraction of CD8+ Tcm cells in atherosclerotic plaques. Correlation is statistically significant at P<0.05. CMV indicates cytomegalovirus; Tcm, central memory T cells.
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jah31695-fig-0012: Correlation coefficient with 95% CI according to Spearman rank test between CMV DNA load and fraction of CD8+ Tcm cells in atherosclerotic plaques. Correlation is statistically significant at P<0.05. CMV indicates cytomegalovirus; Tcm, central memory T cells.

Mentions: Comparison of HHV load and T lymphocyte status revealed a strong positive correlation between the level of CMV DNA in carotid artery plaques and the fraction of intermediately differentiated CD4+ and CD8+ Tem cells (Spearman R=0.642, P=0.004, and R=0.591, P=0.010, respectively) (Figures 9 and 10) as well as the fraction of all CD4+ Tem lymphocytes (Spearman R=0.516, P=0.028). In addition, there was a negative correlation between CMV load and the fraction of CD4+ and CD8+ Tcm lymphocytes (Spearman R=−0.673, P=0.002, and R=0.520, P=0.027, respectively) (Figures 11 and 12).


Cytomegalovirus ‐ Productive Infection Is Associated With Acute Coronary Syndrome
Correlation coefficient with 95% CI according to Spearman rank test between CMV DNA load and fraction of CD8+ Tcm cells in atherosclerotic plaques. Correlation is statistically significant at P<0.05. CMV indicates cytomegalovirus; Tcm, central memory T cells.
© Copyright Policy - creativeCommonsBy-nc
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015295&req=5

jah31695-fig-0012: Correlation coefficient with 95% CI according to Spearman rank test between CMV DNA load and fraction of CD8+ Tcm cells in atherosclerotic plaques. Correlation is statistically significant at P<0.05. CMV indicates cytomegalovirus; Tcm, central memory T cells.
Mentions: Comparison of HHV load and T lymphocyte status revealed a strong positive correlation between the level of CMV DNA in carotid artery plaques and the fraction of intermediately differentiated CD4+ and CD8+ Tem cells (Spearman R=0.642, P=0.004, and R=0.591, P=0.010, respectively) (Figures 9 and 10) as well as the fraction of all CD4+ Tem lymphocytes (Spearman R=0.516, P=0.028). In addition, there was a negative correlation between CMV load and the fraction of CD4+ and CD8+ Tcm lymphocytes (Spearman R=−0.673, P=0.002, and R=0.520, P=0.027, respectively) (Figures 11 and 12).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Although an association between human herpesvirus (HHV) infection and atherosclerosis has been suggested, the data supporting such an association are controversial and, in most cases, are based on serological evidence or on the presence of cell&#8208;associated HHV DNA, which do not report about actual viral replication. We quantified the DNA of all 8 types of HHVs in plasma, in which their presence is evidence of viral replication.

Methods and results: Using quantitative real&#8208;time polymerase chain reaction, we evaluated the presence of HHV DNA in blood samples obtained at the time of hospitalization from 71 patients with acute coronary syndrome, 26 patients with stable coronary artery disease, and 53 healthy volunteers and in atherosclerotic plaques of 22 patients with peripheral artery disease who underwent endarterectomy. HHV&#8208;5 (cytomegalovirus [CMV]) was the only HHV with a level that was higher in acute coronary syndrome patients than in the control group and that correlated with the level of high&#8208;sensitivity C&#8208;reactive protein. The numbers of effector memory T cells positively correlated with the numbers of CMV genome copies in carotid arteries plaques, whereas the numbers of central memory T cells negatively correlated with CMV copy numbers.

Conclusions: Of all HHV levels, only CMV was higher in patients with stable coronary artery disease and acute coronary syndrome than in the healthy group, and its load correlated with the level of high&#8208;sensitivity C&#8208;reactive protein. The level of CMV in atherosclerotic plaques correlated with the state of immunoactivation of lymphocytes in plaques, suggesting that the reactivation of CMV may contribute to the immune activation associated with the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus