Limits...
Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

FTIR spectra of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective temperatures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5015095&req=5

f4: FTIR spectra of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective temperatures.

Mentions: FTIR spectra (Fig. 4) of same powders show presence of hydroxyl (-OH) group around 3445 cm−1, along with Si-O-Si stretching frequency around 465 cm−1 and Si-O-Si bending frequency around 1020 cm−1 for all samples31. Other band assignments included Si-OH symmetric stretch at 780–980 cm−1 and vibrational mode of asymmetric stretch of Si-O-Si between 1100–1000 cm−1. The band assignments are summarized and are given in Table 3.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
FTIR spectra of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective temperatures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f4: FTIR spectra of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective temperatures.
Mentions: FTIR spectra (Fig. 4) of same powders show presence of hydroxyl (-OH) group around 3445 cm−1, along with Si-O-Si stretching frequency around 465 cm−1 and Si-O-Si bending frequency around 1020 cm−1 for all samples31. Other band assignments included Si-OH symmetric stretch at 780–980 cm−1 and vibrational mode of asymmetric stretch of Si-O-Si between 1100–1000 cm−1. The band assignments are summarized and are given in Table 3.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus