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Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

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XRD patters of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective tempertaures.
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f3: XRD patters of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective tempertaures.

Mentions: XRD pattern (Fig. 3) of respective heat treated specimens for each composition confirms amorphous nature with broad diffraction at 2θ ranging between 20–35° indicative of disorder in the structure and glassy nature of powders. Addition of dopants had no appreciable influence on the glassy structure of base material except slight changes in amorphicity and no appearance of any crystalline peak.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
XRD patters of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective tempertaures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f3: XRD patters of (a) BAG, (b) L-BAG, (c) S-BAG and (d) LS-BAG samples heat treated at their respective tempertaures.
Mentions: XRD pattern (Fig. 3) of respective heat treated specimens for each composition confirms amorphous nature with broad diffraction at 2θ ranging between 20–35° indicative of disorder in the structure and glassy nature of powders. Addition of dopants had no appreciable influence on the glassy structure of base material except slight changes in amorphicity and no appearance of any crystalline peak.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus