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Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Fluorochrome labeling study (after oxytetracycline markings) taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
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f16: Fluorochrome labeling study (after oxytetracycline markings) taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.

Mentions: Fig. 16 shows images of samples after oxytetracycline marking, where golden yellow florescence represents new bone and dark sea green indicates matured old bone. After 2 months, BAG scaffolds depicted double tone golden yellow fluorescence in a narrow zone in the defect site and the host bone looked dark sea green homogenous color. Relatively, better intensity of new bone formation (golden yellow fluorescence) was observed in S-BAG and LS-BAG at this time point. L-BAG at this time point also showed more new bone formation as compared to pure sample. At 4 months, all the samples depicted more new bone formation as compared to 2 month. However, distinct new bone formation was exhibited in all doped bioactive glass implants. LS-BAG implanted bone showed wide regions of golden yellow fluorescence (new bone formation) indicating rapid bone regeneration. S-BAG bone samples showed scattered and multiple regions new bone formation in defect area demonstrating their effectiveness in bone regeneration. Based on the calculation, percentage of bone formation through fluorochrome labeling images at two time point of 2 and 4 months have been done and is given in Table 7.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
Fluorochrome labeling study (after oxytetracycline markings) taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f16: Fluorochrome labeling study (after oxytetracycline markings) taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
Mentions: Fig. 16 shows images of samples after oxytetracycline marking, where golden yellow florescence represents new bone and dark sea green indicates matured old bone. After 2 months, BAG scaffolds depicted double tone golden yellow fluorescence in a narrow zone in the defect site and the host bone looked dark sea green homogenous color. Relatively, better intensity of new bone formation (golden yellow fluorescence) was observed in S-BAG and LS-BAG at this time point. L-BAG at this time point also showed more new bone formation as compared to pure sample. At 4 months, all the samples depicted more new bone formation as compared to 2 month. However, distinct new bone formation was exhibited in all doped bioactive glass implants. LS-BAG implanted bone showed wide regions of golden yellow fluorescence (new bone formation) indicating rapid bone regeneration. S-BAG bone samples showed scattered and multiple regions new bone formation in defect area demonstrating their effectiveness in bone regeneration. Based on the calculation, percentage of bone formation through fluorochrome labeling images at two time point of 2 and 4 months have been done and is given in Table 7.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.