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Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Histological sections taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
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f15: Histological sections taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.

Mentions: Fig. 15 and Table 6 show the histological section images and evaluation report of bone-implant interface at 2 and 4 months after observing different cellular events. BAG scaffolds (Fig. 15a) showed well formed bony structure containing haversian system, canaliculi and sinusoidal spaces along with deposition of R.B.C., fat cells and scanty numbers of osteoblast in peri-medullary areas after 2 months. Strontium doped scaffolds (S-BAG: Fig. 15b) at 2 month showed prominent osteoblastic activity characterized by sufficient number of haversian canal, canaliculi, lacunae and osteoblastic cells with suitable cytoplasmic ratios. The bony matrix is invaded by highly proliferative branches of vessels containing sufficient amount of R.B.C, bony progenitor cells and focal calcified points. Similarly, L-BAG scaffolds (Fig. 15c) showed well developed bony structure with robust haversian system, osseous canaliculi and bony plates. The LS-BAG samples (Fig. 15d) depicted well formed osseous structure containing haversian canal, lamellae and canaliculi which was invaded by numerous blood vessels along with prominent osteoblastic and osteoclastic activities in the margin of lesion.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
Histological sections taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f15: Histological sections taken after 2 and 4 months post-operatively implanted with (a) BAG, (b) S-BAG, (c) L-BAG and (d) LS-BAG.
Mentions: Fig. 15 and Table 6 show the histological section images and evaluation report of bone-implant interface at 2 and 4 months after observing different cellular events. BAG scaffolds (Fig. 15a) showed well formed bony structure containing haversian system, canaliculi and sinusoidal spaces along with deposition of R.B.C., fat cells and scanty numbers of osteoblast in peri-medullary areas after 2 months. Strontium doped scaffolds (S-BAG: Fig. 15b) at 2 month showed prominent osteoblastic activity characterized by sufficient number of haversian canal, canaliculi, lacunae and osteoblastic cells with suitable cytoplasmic ratios. The bony matrix is invaded by highly proliferative branches of vessels containing sufficient amount of R.B.C, bony progenitor cells and focal calcified points. Similarly, L-BAG scaffolds (Fig. 15c) showed well developed bony structure with robust haversian system, osseous canaliculi and bony plates. The LS-BAG samples (Fig. 15d) depicted well formed osseous structure containing haversian canal, lamellae and canaliculi which was invaded by numerous blood vessels along with prominent osteoblastic and osteoclastic activities in the margin of lesion.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.