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Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Serial slices along the Z-axis in micro-CT.Images are obtained for implanted L-BAG scaffolds. (a) After 2 months; (b) After 4 months.
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f11: Serial slices along the Z-axis in micro-CT.Images are obtained for implanted L-BAG scaffolds. (a) After 2 months; (b) After 4 months.

Mentions: Serial slices of X rays were carried out throughout Z-axis of a particular implanted bone section, images thus obtained were clubbed together and are given in Fig. 10a,b for BAG, 11a,b for L-BAG, 12a,b for S-BAG and 13a,b for LS-BAG after 2 and 4 months respectively. Serial images for BAG taken after 2 and 4 months showed that the porous scaffold has started degrading as revealed after 4 months but maintained its structure after 2 months. From the grey scale quantification it can be shown that BAG samples had higher amount of mature bone tissue after 4 months than the 2 months when more soft tissue apposition was evident. Stability of the implant thus impaired after 4 months which is anticipated to be continued and simultaneously converted to hard cortical tissue. Effect of lithium and strontium can be an interesting parameter which can dictate the degree of bone tissue conversion with time.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
Serial slices along the Z-axis in micro-CT.Images are obtained for implanted L-BAG scaffolds. (a) After 2 months; (b) After 4 months.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f11: Serial slices along the Z-axis in micro-CT.Images are obtained for implanted L-BAG scaffolds. (a) After 2 months; (b) After 4 months.
Mentions: Serial slices of X rays were carried out throughout Z-axis of a particular implanted bone section, images thus obtained were clubbed together and are given in Fig. 10a,b for BAG, 11a,b for L-BAG, 12a,b for S-BAG and 13a,b for LS-BAG after 2 and 4 months respectively. Serial images for BAG taken after 2 and 4 months showed that the porous scaffold has started degrading as revealed after 4 months but maintained its structure after 2 months. From the grey scale quantification it can be shown that BAG samples had higher amount of mature bone tissue after 4 months than the 2 months when more soft tissue apposition was evident. Stability of the implant thus impaired after 4 months which is anticipated to be continued and simultaneously converted to hard cortical tissue. Effect of lithium and strontium can be an interesting parameter which can dictate the degree of bone tissue conversion with time.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.