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Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.


Surgical placement of the porous scaffolds (with or without doped BAG).
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f1: Surgical placement of the porous scaffolds (with or without doped BAG).

Mentions: The animal experiments were performed following an ethical committee approved protocol in accordance with Institutional Animal Ethical Committee (IAEC), West Bengal University of Animal and Fishery Sciences (WBUAFS), West Bengal, India (Permit No. Pharma/IAEC/34 dated 30 June 2014). Sixteen adult New Zealand White rabbits (1.5–2 kg) were randomized into four groups (n = 4): control group I (pure BAG) and the test animals, group II (S-BAG), group III (L-BAG) and group IV (LS-BAG) with bilateral implantation. All surgeries were performed under general intramuscular anesthesia using xylazine hydrochloride (6 mg/kg) (Xylaxin, Indian Immunologicals, India) and ketamine hydrochloride (33 mg/kg) (Ketalar, Parke-Davis, India). Scaffolds were press fitted within the created defects in the distal metaphyseal region of femur and wounds were sutured in three layers (Fig. 1). Subsequently, animals were administered with cefotaxime sodium (Mapra India, India) at 20 mg/kg body weight intramuscularly for 5 days twice daily at 12 h interval and meloxicam at 0.2 mL (Intas Pharmaceuticals, India) once daily. Animals were finally sacrificed after 2 and 4 months of implantation.


Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds
Surgical placement of the porous scaffolds (with or without doped BAG).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015095&req=5

f1: Surgical placement of the porous scaffolds (with or without doped BAG).
Mentions: The animal experiments were performed following an ethical committee approved protocol in accordance with Institutional Animal Ethical Committee (IAEC), West Bengal University of Animal and Fishery Sciences (WBUAFS), West Bengal, India (Permit No. Pharma/IAEC/34 dated 30 June 2014). Sixteen adult New Zealand White rabbits (1.5–2 kg) were randomized into four groups (n = 4): control group I (pure BAG) and the test animals, group II (S-BAG), group III (L-BAG) and group IV (LS-BAG) with bilateral implantation. All surgeries were performed under general intramuscular anesthesia using xylazine hydrochloride (6 mg/kg) (Xylaxin, Indian Immunologicals, India) and ketamine hydrochloride (33 mg/kg) (Ketalar, Parke-Davis, India). Scaffolds were press fitted within the created defects in the distal metaphyseal region of femur and wounds were sutured in three layers (Fig. 1). Subsequently, animals were administered with cefotaxime sodium (Mapra India, India) at 20 mg/kg body weight intramuscularly for 5 days twice daily at 12 h interval and meloxicam at 0.2 mL (Intas Pharmaceuticals, India) once daily. Animals were finally sacrificed after 2 and 4 months of implantation.

View Article: PubMed Central - PubMed

ABSTRACT

Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.

No MeSH data available.