Limits...
Micro RNA-98 interferes with expression interleukin-10 in peripheral B cells of patients with lung cancer

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin (IL)-10-producing B cells (B10 cells) plays an important role in the tumor tolerance. High frequency of peripheral B10 cell was reported in patients with lung cancer recently. Micro RNA (miR) regulates some gene expression. This study test a hypothesis that miR-98 suppresses the expression of IL-10 in B cells of subjects with lung cancer. The results showed that the levels of miR-98 were significantly less in peripheral B cells of patients with lung cancer than that in healthy subjects. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients as compared with healthy controls. A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. Serum IL-13 was higher in lung cancer patients than that in healthy controls. The levels of IL-13 were also negatively correlated with IL-10 in B cells. Exposure B10 cells to IL-13 in the culture or over expression of miR-98 reduced the expression of IL-10 in B cells. Administration with miR-98-laden liposomes inhibited the lung cancer growth in a mouse model. In conclusion, up regulation of miR-98 inhibits the expression of IL-10 in B cells, which may contribute to inhibit the lung cancer tolerance in the body.

No MeSH data available.


Related in: MedlinePlus

MiR-98-carrying liposomes inhibit B10 cells and suppress tumor growth.(A) the scatter plots show the tumor size in individual mice on day 12. (B) the scatter plots show the frequency of peripheral B10 cells. (C) the gated dot plots show the representative results of panel B. Each group consists of 10 mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5015073&req=5

f4: MiR-98-carrying liposomes inhibit B10 cells and suppress tumor growth.(A) the scatter plots show the tumor size in individual mice on day 12. (B) the scatter plots show the frequency of peripheral B10 cells. (C) the gated dot plots show the representative results of panel B. Each group consists of 10 mice.

Mentions: Since B10 cells play an important role in the cancer-tolerance that contributes cancer cells to escape from the immune surveillance8, to inhibit IL-10 expression in B cells may inhibit cancer growth. To test this, we transplanted lung cancer cells to mice to induce a cancer mass. The cancer-bearing mice were intraperitoneal injection with miR-98 liposomes three times with 3 days apart from each injection. The cancer size was measured daily for 12 days. The results showed that the administration with miR-98 liposomes significantly inhibited the cancer growth (Fig. 4A). We also assessed the frequency of B10 cells in the peripheral blood that we collected at the sacrifice on day 12. The results showed that the frequency of B10 cells was significantly higher in cancer-bearing mice treated with saline than that of naive control mice. Administration with miR-98 liposomes significantly decreased the B10 cell frequency in cancer-bearing mice (Fig. 4B,C). The results demonstrate that administration with miR-98-laden liposomes can inhibit lung cancer growth in mice via suppression of IL-10 expression in B cells.


Micro RNA-98 interferes with expression interleukin-10 in peripheral B cells of patients with lung cancer
MiR-98-carrying liposomes inhibit B10 cells and suppress tumor growth.(A) the scatter plots show the tumor size in individual mice on day 12. (B) the scatter plots show the frequency of peripheral B10 cells. (C) the gated dot plots show the representative results of panel B. Each group consists of 10 mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015073&req=5

f4: MiR-98-carrying liposomes inhibit B10 cells and suppress tumor growth.(A) the scatter plots show the tumor size in individual mice on day 12. (B) the scatter plots show the frequency of peripheral B10 cells. (C) the gated dot plots show the representative results of panel B. Each group consists of 10 mice.
Mentions: Since B10 cells play an important role in the cancer-tolerance that contributes cancer cells to escape from the immune surveillance8, to inhibit IL-10 expression in B cells may inhibit cancer growth. To test this, we transplanted lung cancer cells to mice to induce a cancer mass. The cancer-bearing mice were intraperitoneal injection with miR-98 liposomes three times with 3 days apart from each injection. The cancer size was measured daily for 12 days. The results showed that the administration with miR-98 liposomes significantly inhibited the cancer growth (Fig. 4A). We also assessed the frequency of B10 cells in the peripheral blood that we collected at the sacrifice on day 12. The results showed that the frequency of B10 cells was significantly higher in cancer-bearing mice treated with saline than that of naive control mice. Administration with miR-98 liposomes significantly decreased the B10 cell frequency in cancer-bearing mice (Fig. 4B,C). The results demonstrate that administration with miR-98-laden liposomes can inhibit lung cancer growth in mice via suppression of IL-10 expression in B cells.

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin (IL)-10-producing B cells (B10 cells) plays an important role in the tumor tolerance. High frequency of peripheral B10 cell was reported in patients with lung cancer recently. Micro RNA (miR) regulates some gene expression. This study test a hypothesis that miR-98 suppresses the expression of IL-10 in B cells of subjects with lung cancer. The results showed that the levels of miR-98 were significantly less in peripheral B cells of patients with lung cancer than that in healthy subjects. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients as compared with healthy controls. A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. Serum IL-13 was higher in lung cancer patients than that in healthy controls. The levels of IL-13 were also negatively correlated with IL-10 in B cells. Exposure B10 cells to IL-13 in the culture or over expression of miR-98 reduced the expression of IL-10 in B cells. Administration with miR-98-laden liposomes inhibited the lung cancer growth in a mouse model. In conclusion, up regulation of miR-98 inhibits the expression of IL-10 in B cells, which may contribute to inhibit the lung cancer tolerance in the body.

No MeSH data available.


Related in: MedlinePlus