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Micro RNA-98 interferes with expression interleukin-10 in peripheral B cells of patients with lung cancer

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin (IL)-10-producing B cells (B10 cells) plays an important role in the tumor tolerance. High frequency of peripheral B10 cell was reported in patients with lung cancer recently. Micro RNA (miR) regulates some gene expression. This study test a hypothesis that miR-98 suppresses the expression of IL-10 in B cells of subjects with lung cancer. The results showed that the levels of miR-98 were significantly less in peripheral B cells of patients with lung cancer than that in healthy subjects. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients as compared with healthy controls. A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. Serum IL-13 was higher in lung cancer patients than that in healthy controls. The levels of IL-13 were also negatively correlated with IL-10 in B cells. Exposure B10 cells to IL-13 in the culture or over expression of miR-98 reduced the expression of IL-10 in B cells. Administration with miR-98-laden liposomes inhibited the lung cancer growth in a mouse model. In conclusion, up regulation of miR-98 inhibits the expression of IL-10 in B cells, which may contribute to inhibit the lung cancer tolerance in the body.

No MeSH data available.


Related in: MedlinePlus

Peripheral B10 cells are more in patients with lung cancer.(A) the gated dot plots indicate the frequency of B10 cells in PBMCs of healthy subjects (n = 10) and patients with lung cancer (n = 10). (B) the dot plots show the individual value distribution of panel A.
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f1: Peripheral B10 cells are more in patients with lung cancer.(A) the gated dot plots indicate the frequency of B10 cells in PBMCs of healthy subjects (n = 10) and patients with lung cancer (n = 10). (B) the dot plots show the individual value distribution of panel A.

Mentions: B10 cells play a role in the tumor tolerance14. To understand the role of B10 cells in the pathogenesis of lung cancer, we collected the peripheral blood samples from NSCLC patients. PBMCs were isolated from the blood samples and analyzed by flow cytometry. The results showed that higher frequency of B10 cells (14.2%) was detected in the cancer group than that in the healthy control group (2.74%) (Fig. 1). The data demonstrate that B10 cells are increased in patients with lung cancer.


Micro RNA-98 interferes with expression interleukin-10 in peripheral B cells of patients with lung cancer
Peripheral B10 cells are more in patients with lung cancer.(A) the gated dot plots indicate the frequency of B10 cells in PBMCs of healthy subjects (n = 10) and patients with lung cancer (n = 10). (B) the dot plots show the individual value distribution of panel A.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015073&req=5

f1: Peripheral B10 cells are more in patients with lung cancer.(A) the gated dot plots indicate the frequency of B10 cells in PBMCs of healthy subjects (n = 10) and patients with lung cancer (n = 10). (B) the dot plots show the individual value distribution of panel A.
Mentions: B10 cells play a role in the tumor tolerance14. To understand the role of B10 cells in the pathogenesis of lung cancer, we collected the peripheral blood samples from NSCLC patients. PBMCs were isolated from the blood samples and analyzed by flow cytometry. The results showed that higher frequency of B10 cells (14.2%) was detected in the cancer group than that in the healthy control group (2.74%) (Fig. 1). The data demonstrate that B10 cells are increased in patients with lung cancer.

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin (IL)-10-producing B cells (B10 cells) plays an important role in the tumor tolerance. High frequency of peripheral B10 cell was reported in patients with lung cancer recently. Micro RNA (miR) regulates some gene expression. This study test a hypothesis that miR-98 suppresses the expression of IL-10 in B cells of subjects with lung cancer. The results showed that the levels of miR-98 were significantly less in peripheral B cells of patients with lung cancer than that in healthy subjects. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients as compared with healthy controls. A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. Serum IL-13 was higher in lung cancer patients than that in healthy controls. The levels of IL-13 were also negatively correlated with IL-10 in B cells. Exposure B10 cells to IL-13 in the culture or over expression of miR-98 reduced the expression of IL-10 in B cells. Administration with miR-98-laden liposomes inhibited the lung cancer growth in a mouse model. In conclusion, up regulation of miR-98 inhibits the expression of IL-10 in B cells, which may contribute to inhibit the lung cancer tolerance in the body.

No MeSH data available.


Related in: MedlinePlus