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Genome-wide Identification of TCP Family Transcription Factors from Populus euphratica and Their Involvement in Leaf Shape Regulation

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ABSTRACT

Higher plants have been shown to experience a juvenile vegetative phase, an adult vegetative phase, and a reproductive phase during its postembryonic development and distinct lateral organ morphologies have been observed at the different development stages. Populus euphratica, commonly known as a desert poplar, has developed heteromorphic leaves during its development. The TCP family genes encode a group of plant-specific transcription factors involved in several aspects of plant development. In particular, TCPs have been shown to influence leaf size and shape in many herbaceous plants. However, whether these functions are conserved in woody plants remains unknown. In the present study, we carried out genome-wide identification of TCP genes in P. euphratica and P. trichocarpa, and 33 and 36 genes encoding putative TCP proteins were found, respectively. Phylogenetic analysis of the poplar TCPs together with Arabidopsis TCPs indicated a biased expansion of the TCP gene family via segmental duplications. In addition, our results have also shown a correlation between different expression patterns of several P. euphratica TCP genes and leaf shape variations, indicating their involvement in the regulation of leaf shape development.

No MeSH data available.


Genomic structure and motif composition of poplar TCPs.(A) Phylogenetic tree of P. trichocarpa and P. euphratica TCP proteins. (B) Genomic structure of poplar TCP genes. Exons, introns, and UTRs are indicated with yellow boxes, blue boxes and black lines, respectively. (C) Motif composition of poplar TCP proteins. Conserved motifs in the poplar TCP proteins are indicated by colored boxes.
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f3: Genomic structure and motif composition of poplar TCPs.(A) Phylogenetic tree of P. trichocarpa and P. euphratica TCP proteins. (B) Genomic structure of poplar TCP genes. Exons, introns, and UTRs are indicated with yellow boxes, blue boxes and black lines, respectively. (C) Motif composition of poplar TCP proteins. Conserved motifs in the poplar TCP proteins are indicated by colored boxes.

Mentions: As shown in Fig. 3B, most (53 out of 69) of the poplar TCP genes contain only one exon and 11 members contain one intron and two exons. Only PeuTCP18 compromises two introns and three exons and PtrTCP6 possesses three introns and four exons, whereas three genes (PeuTCP17, PtrTCP29 and PeuTCP8) consist of four introns and five exons. Moreover, similar exon/intron structures were found in poplar genes within the same phylogenetic subfamily, further confirming the reliability of our phylogenetic analysis.


Genome-wide Identification of TCP Family Transcription Factors from Populus euphratica and Their Involvement in Leaf Shape Regulation
Genomic structure and motif composition of poplar TCPs.(A) Phylogenetic tree of P. trichocarpa and P. euphratica TCP proteins. (B) Genomic structure of poplar TCP genes. Exons, introns, and UTRs are indicated with yellow boxes, blue boxes and black lines, respectively. (C) Motif composition of poplar TCP proteins. Conserved motifs in the poplar TCP proteins are indicated by colored boxes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5015053&req=5

f3: Genomic structure and motif composition of poplar TCPs.(A) Phylogenetic tree of P. trichocarpa and P. euphratica TCP proteins. (B) Genomic structure of poplar TCP genes. Exons, introns, and UTRs are indicated with yellow boxes, blue boxes and black lines, respectively. (C) Motif composition of poplar TCP proteins. Conserved motifs in the poplar TCP proteins are indicated by colored boxes.
Mentions: As shown in Fig. 3B, most (53 out of 69) of the poplar TCP genes contain only one exon and 11 members contain one intron and two exons. Only PeuTCP18 compromises two introns and three exons and PtrTCP6 possesses three introns and four exons, whereas three genes (PeuTCP17, PtrTCP29 and PeuTCP8) consist of four introns and five exons. Moreover, similar exon/intron structures were found in poplar genes within the same phylogenetic subfamily, further confirming the reliability of our phylogenetic analysis.

View Article: PubMed Central - PubMed

ABSTRACT

Higher plants have been shown to experience a juvenile vegetative phase, an adult vegetative phase, and a reproductive phase during its postembryonic development and distinct lateral organ morphologies have been observed at the different development stages. Populus euphratica, commonly known as a desert poplar, has developed heteromorphic leaves during its development. The TCP family genes encode a group of plant-specific transcription factors involved in several aspects of plant development. In particular, TCPs have been shown to influence leaf size and shape in many herbaceous plants. However, whether these functions are conserved in woody plants remains unknown. In the present study, we carried out genome-wide identification of TCP genes in P. euphratica and P. trichocarpa, and 33 and 36 genes encoding putative TCP proteins were found, respectively. Phylogenetic analysis of the poplar TCPs together with Arabidopsis TCPs indicated a biased expansion of the TCP gene family via segmental duplications. In addition, our results have also shown a correlation between different expression patterns of several P. euphratica TCP genes and leaf shape variations, indicating their involvement in the regulation of leaf shape development.

No MeSH data available.