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Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia

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ABSTRACT

To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX.

No MeSH data available.


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Effect of PTX on testes histopathology. (a) Representative micrographs of hematoxylin-eosin-stained sections in the testes of rats, 400x, insert bar = 100 μm. (b) Quantification of Johnson's score of testes injuries. Control group (n = 8); intermittent hypobaric hypoxia (IHH) group (n = 8); pentoxifylline (PTX)+IHH group (n = 8). Data were presented as mean ± standard deviation (SD). ∗p < 0.05 compared with IHH+Vehicle.
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fig3: Effect of PTX on testes histopathology. (a) Representative micrographs of hematoxylin-eosin-stained sections in the testes of rats, 400x, insert bar = 100 μm. (b) Quantification of Johnson's score of testes injuries. Control group (n = 8); intermittent hypobaric hypoxia (IHH) group (n = 8); pentoxifylline (PTX)+IHH group (n = 8). Data were presented as mean ± standard deviation (SD). ∗p < 0.05 compared with IHH+Vehicle.

Mentions: IHH induced significant histopathological changes, including disordered arrangement and exfoliation of seminiferous epithelium cells, and reduced Johnsen's scores. Johnsen's scores were 9.66 ± 1.17, 7.47 ± 1.52, and 8.93 ± 1.49 for Control, IHH+Vehicle, and PTX+IHH, respectively. There was a significant difference between the Control and IHH+Vehicle groups (p < 0.05). Importantly, administration of PTX improved the above indicators (Figure 3).


Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
Effect of PTX on testes histopathology. (a) Representative micrographs of hematoxylin-eosin-stained sections in the testes of rats, 400x, insert bar = 100 μm. (b) Quantification of Johnson's score of testes injuries. Control group (n = 8); intermittent hypobaric hypoxia (IHH) group (n = 8); pentoxifylline (PTX)+IHH group (n = 8). Data were presented as mean ± standard deviation (SD). ∗p < 0.05 compared with IHH+Vehicle.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5015030&req=5

fig3: Effect of PTX on testes histopathology. (a) Representative micrographs of hematoxylin-eosin-stained sections in the testes of rats, 400x, insert bar = 100 μm. (b) Quantification of Johnson's score of testes injuries. Control group (n = 8); intermittent hypobaric hypoxia (IHH) group (n = 8); pentoxifylline (PTX)+IHH group (n = 8). Data were presented as mean ± standard deviation (SD). ∗p < 0.05 compared with IHH+Vehicle.
Mentions: IHH induced significant histopathological changes, including disordered arrangement and exfoliation of seminiferous epithelium cells, and reduced Johnsen's scores. Johnsen's scores were 9.66 ± 1.17, 7.47 ± 1.52, and 8.93 ± 1.49 for Control, IHH+Vehicle, and PTX+IHH, respectively. There was a significant difference between the Control and IHH+Vehicle groups (p < 0.05). Importantly, administration of PTX improved the above indicators (Figure 3).

View Article: PubMed Central - PubMed

ABSTRACT

To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96&thinsp;h of hypobaric hypoxia followed by 96&thinsp;h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX.

No MeSH data available.


Related in: MedlinePlus