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The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed ( Nigella sativa L.) Oil

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ABSTRACT

The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

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Thymoquinone concentration of Nigella sativa oil as determined by HPLC: (a) 490 supercritical fluid extraction, (b) cold press extraction, and (c) thymoquinone standard.
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fig2: Thymoquinone concentration of Nigella sativa oil as determined by HPLC: (a) 490 supercritical fluid extraction, (b) cold press extraction, and (c) thymoquinone standard.

Mentions: The HPLC analysis showed that the two oil samples (SFE and cold pressed) contained thymoquinone TQ (Figures 2(a) and 2(b)). The highest amount of TQ was observed in the SFE oil and was 6.63 mg/mL of oil, while the concentration of TQ was 1.56 mg/mL oil for the cold press sample (Table 4). The methods of extraction showed important differences (p > 0.05) for the SFE with higher thymoquinone quantity.


The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed ( Nigella sativa L.) Oil
Thymoquinone concentration of Nigella sativa oil as determined by HPLC: (a) 490 supercritical fluid extraction, (b) cold press extraction, and (c) thymoquinone standard.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5015008&req=5

fig2: Thymoquinone concentration of Nigella sativa oil as determined by HPLC: (a) 490 supercritical fluid extraction, (b) cold press extraction, and (c) thymoquinone standard.
Mentions: The HPLC analysis showed that the two oil samples (SFE and cold pressed) contained thymoquinone TQ (Figures 2(a) and 2(b)). The highest amount of TQ was observed in the SFE oil and was 6.63 mg/mL of oil, while the concentration of TQ was 1.56 mg/mL oil for the cold press sample (Table 4). The methods of extraction showed important differences (p > 0.05) for the SFE with higher thymoquinone quantity.

View Article: PubMed Central - PubMed

ABSTRACT

The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

No MeSH data available.


Related in: MedlinePlus