Limits...
Matrix metalloproteinase-9 and -2 and tissue inhibitor of matrix metalloproteinase-2 in invasive pituitary adenomas

View Article: PubMed Central - PubMed

ABSTRACT

The extracellular matrix is important for tumor invasion and metastasis. Normal function of the extracellular matrix depends on the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). The objective of this meta-analysis was to assess the relationship between expression of MMP-9, MMP-2, and TIMP-2 and invasion of pituitary adenomas.

We searched Pubmed, Embase, and the Chinese Biomedical Database up to October 2015. RevMan 5.1 software (Cochrane Collaboration, Copenhagen, Denmark) was used for statistical analysis. We calculated the standardized mean difference (SMD) for data expressed as mean ± standard deviation because of the difference in the detection method.

Twenty-four studies (1320 patients) were included. MMP-9 expression was higher in the patients with invasive pituitary adenomas (IPAs) than patients with noninvasive pituitary adenomas (NIPAs) with detection methods of IHC [odds ratio (OR) = 5.48, 95% confidence interval (CI) = 2.61–11.50, P < 0.00001), and reverse transcriptase-polymerase chain reaction (SMD = 2.28, 95% CI = 0.91–3.64, P = 0.001). MMP-2 expression was also increased in patients with IPAs at the protein level (OR = 3.58, 95% CI = 1.63–7.87, P = 0.001), and RNA level (SMD = 3.91, 95% CI = 1.52–6.29, P = 0.001). Meta-analysis showed that there was no difference in TIMP-2 expression between invasive and NIPAs at the protein level (OR = 0.38, 95% CI = 0.06–2.26, P = 0.29). MMP-9 expression in prolactinomas and nonfunctioning pituitary adenomas was also no difference (OR = 1.03, 95% CI = 0.48–2.20, P = 0.95).

The results indicated that MMP-9 and -2 may be correlated with invasiveness of pituitary adenomas, although their relationship with functional status of pituitary adenomas is still not clear. TIMP-2 expression in IPAs needs to be investigated further.

No MeSH data available.


Related in: MedlinePlus

Forest plots for the relationship between TIMP-2 expression and tumor invasiveness of PAs at the protein level. M-H = Mantel–Haenszel test, Random = a random effects model, CI = confidence intervals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4998479&req=5

Figure 4: Forest plots for the relationship between TIMP-2 expression and tumor invasiveness of PAs at the protein level. M-H = Mantel–Haenszel test, Random = a random effects model, CI = confidence intervals.

Mentions: Because of the considerable heterogeneity, the random effects model was also used for this meta-analysis. Five articles (189 IPAs and 115 NIPAs) reported TIMP-2 expression, but P = 0.29 (OR = 0.38, 95% CI = 0.06–2.26; Fig. 4) demonstrated that there was no statistical significance in TIMP-2 expression between IPAs and NIPAs. The results of sensitivity analysis showed that the study of Gültekin et al influenced the pooled OR greatly, indicating that this article may have been one of the sources of the substantial heterogeneity (Supplemental Table S6). Funnel plot were derived for MMP-9 expression in IPAs and NIPA at the protein and RNA levels and MMP-2 expression in IPAs and NIPA at the protein level. The symmetrical distribution funnel plot visually suggested that there was no publication bias (Figs. 6A and B, and 7), and the result of Begg's test also indicated no publication bias (data were not shown).


Matrix metalloproteinase-9 and -2 and tissue inhibitor of matrix metalloproteinase-2 in invasive pituitary adenomas
Forest plots for the relationship between TIMP-2 expression and tumor invasiveness of PAs at the protein level. M-H = Mantel–Haenszel test, Random = a random effects model, CI = confidence intervals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998479&req=5

Figure 4: Forest plots for the relationship between TIMP-2 expression and tumor invasiveness of PAs at the protein level. M-H = Mantel–Haenszel test, Random = a random effects model, CI = confidence intervals.
Mentions: Because of the considerable heterogeneity, the random effects model was also used for this meta-analysis. Five articles (189 IPAs and 115 NIPAs) reported TIMP-2 expression, but P = 0.29 (OR = 0.38, 95% CI = 0.06–2.26; Fig. 4) demonstrated that there was no statistical significance in TIMP-2 expression between IPAs and NIPAs. The results of sensitivity analysis showed that the study of Gültekin et al influenced the pooled OR greatly, indicating that this article may have been one of the sources of the substantial heterogeneity (Supplemental Table S6). Funnel plot were derived for MMP-9 expression in IPAs and NIPA at the protein and RNA levels and MMP-2 expression in IPAs and NIPA at the protein level. The symmetrical distribution funnel plot visually suggested that there was no publication bias (Figs. 6A and B, and 7), and the result of Begg's test also indicated no publication bias (data were not shown).

View Article: PubMed Central - PubMed

ABSTRACT

The extracellular matrix is important for tumor invasion and metastasis. Normal function of the extracellular matrix depends on the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). The objective of this meta-analysis was to assess the relationship between expression of MMP-9, MMP-2, and TIMP-2 and invasion of pituitary adenomas.

We searched Pubmed, Embase, and the Chinese Biomedical Database up to October 2015. RevMan 5.1 software (Cochrane Collaboration, Copenhagen, Denmark) was used for statistical analysis. We calculated the standardized mean difference (SMD) for data expressed as mean ± standard deviation because of the difference in the detection method.

Twenty-four studies (1320 patients) were included. MMP-9 expression was higher in the patients with invasive pituitary adenomas (IPAs) than patients with noninvasive pituitary adenomas (NIPAs) with detection methods of IHC [odds ratio (OR) = 5.48, 95% confidence interval (CI) = 2.61–11.50, P < 0.00001), and reverse transcriptase-polymerase chain reaction (SMD = 2.28, 95% CI = 0.91–3.64, P = 0.001). MMP-2 expression was also increased in patients with IPAs at the protein level (OR = 3.58, 95% CI = 1.63–7.87, P = 0.001), and RNA level (SMD = 3.91, 95% CI = 1.52–6.29, P = 0.001). Meta-analysis showed that there was no difference in TIMP-2 expression between invasive and NIPAs at the protein level (OR = 0.38, 95% CI = 0.06–2.26, P = 0.29). MMP-9 expression in prolactinomas and nonfunctioning pituitary adenomas was also no difference (OR = 1.03, 95% CI = 0.48–2.20, P = 0.95).

The results indicated that MMP-9 and -2 may be correlated with invasiveness of pituitary adenomas, although their relationship with functional status of pituitary adenomas is still not clear. TIMP-2 expression in IPAs needs to be investigated further.

No MeSH data available.


Related in: MedlinePlus