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Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50–60, 60–80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-β-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-β-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-β-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-β-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.


Related in: MedlinePlus

Proportion of SA-β-gal+ HLECs from different cataract subtypes. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of SA-β-gal+ HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
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Figure 6: Proportion of SA-β-gal+ HLECs from different cataract subtypes. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of SA-β-gal+ HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.

Mentions: To further investigate the relationship between the aging of HLECs and cataract subtypes, the proportion of SA-β-gal+ cells in each subtype and grade were analyzed. The proportion of SA-β-gal+ cells were 13.17% ± 2.00% in grade I, increased to 35.18% ± 4.67% in grade II and 39.87% ± 4.81% in grade III, and finally reached 43.09% ± 4.65% in grade IV and 36.23% ± 2.99% in grade V of cortical cataracts (Fig. 6). These results were consistent with those of senescent HLECs, thus further confirming the influence of the aging of HLECs in the severity of cortical cataracts. The number of SA-β-gal+ cells in each grade of posterior subcapsular cataract was also analyzed. Surprizingly, the severity of posterior subcapsular cataracts could also be influenced by SA-β-gal+ HLECs. The proportion of SA-β-gal+ cells within HLECs were 27.28% ± 7.95% in grade 0, increased to 35.33% ± 3.88% in grade I, and reached the highest at 45.16% ± 6.01% in grade II of posterior subcapsular cataracts (Fig. 6). However, no significant difference was observed among the groups. Similar results to those of senescent HLECs were found for nuclear opalescence (N) or nuclear color (NC) (Fig. 6).


Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans
Proportion of SA-β-gal+ HLECs from different cataract subtypes. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of SA-β-gal+ HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998311&req=5

Figure 6: Proportion of SA-β-gal+ HLECs from different cataract subtypes. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of SA-β-gal+ HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
Mentions: To further investigate the relationship between the aging of HLECs and cataract subtypes, the proportion of SA-β-gal+ cells in each subtype and grade were analyzed. The proportion of SA-β-gal+ cells were 13.17% ± 2.00% in grade I, increased to 35.18% ± 4.67% in grade II and 39.87% ± 4.81% in grade III, and finally reached 43.09% ± 4.65% in grade IV and 36.23% ± 2.99% in grade V of cortical cataracts (Fig. 6). These results were consistent with those of senescent HLECs, thus further confirming the influence of the aging of HLECs in the severity of cortical cataracts. The number of SA-β-gal+ cells in each grade of posterior subcapsular cataract was also analyzed. Surprizingly, the severity of posterior subcapsular cataracts could also be influenced by SA-β-gal+ HLECs. The proportion of SA-β-gal+ cells within HLECs were 27.28% ± 7.95% in grade 0, increased to 35.33% ± 3.88% in grade I, and reached the highest at 45.16% ± 6.01% in grade II of posterior subcapsular cataracts (Fig. 6). However, no significant difference was observed among the groups. Similar results to those of senescent HLECs were found for nuclear opalescence (N) or nuclear color (NC) (Fig. 6).

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50&ndash;60, 60&ndash;80, and &gt;80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-&beta;-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-&beta;-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-&beta;-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-&beta;-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.


Related in: MedlinePlus