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Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50–60, 60–80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-β-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-β-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-β-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-β-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.


Related in: MedlinePlus

Proportion of senescent HLECs from different subtypes of cataracts. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of senescent HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
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Figure 5: Proportion of senescent HLECs from different subtypes of cataracts. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of senescent HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.

Mentions: Many mechanisms for ARC, including the aging of HLECs, have been investigated, but the relationship between the aging of HLECs and cataract grades remains unknown.[2] Therefore, the correlation between the severity of different ARC subtypes and the proportion of senescent cells within HLECs was analyzed. All samples were divided into groups according to cataract severity based on the Lens Opacities Classification System III in terms of 4 features: cortical cataracts (C), nuclear opalescence (N), posterior subcapsular cataracts (P), and nuclear color (NC) (Table 2).[19] The results showed that proportion of senescent cells within HLECs increased with the grade of cortical cataracts. The proportion of senescent cells within HLECs was found to be 19.27% ± 4.83% in grade I, increased to 32.31% ± 4.99% in grade II and 32.67% ± 2.81% in grade III, and finally reached 38.86% ± 5.91% in grade IV and 38.57% ± 3.90% in grade V (Fig. 5). No relevance was found between senescent HLECs and nuclear opalescence (N), nuclear color (NC), or posterior subcapsular cataracts (P) (Fig. 5).


Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans
Proportion of senescent HLECs from different subtypes of cataracts. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of senescent HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998311&req=5

Figure 5: Proportion of senescent HLECs from different subtypes of cataracts. C is short for cortical cataracts, N for nuclear opalescence, P for posterior subcapsular cataract, and NC for nuclear color. Different bars show the different grades of each cataract subtype. Data are presented as mean ± SEM. ∗P < 0.05 versus proportion of senescent HLECs from the group of cortical cataracts grade 1. HLEC = human lens epithelial cell, SEM = standard error of mean.
Mentions: Many mechanisms for ARC, including the aging of HLECs, have been investigated, but the relationship between the aging of HLECs and cataract grades remains unknown.[2] Therefore, the correlation between the severity of different ARC subtypes and the proportion of senescent cells within HLECs was analyzed. All samples were divided into groups according to cataract severity based on the Lens Opacities Classification System III in terms of 4 features: cortical cataracts (C), nuclear opalescence (N), posterior subcapsular cataracts (P), and nuclear color (NC) (Table 2).[19] The results showed that proportion of senescent cells within HLECs increased with the grade of cortical cataracts. The proportion of senescent cells within HLECs was found to be 19.27% ± 4.83% in grade I, increased to 32.31% ± 4.99% in grade II and 32.67% ± 2.81% in grade III, and finally reached 38.86% ± 5.91% in grade IV and 38.57% ± 3.90% in grade V (Fig. 5). No relevance was found between senescent HLECs and nuclear opalescence (N), nuclear color (NC), or posterior subcapsular cataracts (P) (Fig. 5).

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50&ndash;60, 60&ndash;80, and &gt;80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-&beta;-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-&beta;-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-&beta;-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-&beta;-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.


Related in: MedlinePlus