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Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50–60, 60–80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-β-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-β-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-β-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-β-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.


Expressions of Ki67, Sox2, and Abcg2 in the cultured primary human lens epithelial cells (HLECs) from patients older than 60 years. Ki67+, Sox2+, and Abcg2+ cells were not observed in the cultured primary HLECs from patients older than 60 years, representative pictures showed as A–B. Scale bar, 40 μm.
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Figure 2: Expressions of Ki67, Sox2, and Abcg2 in the cultured primary human lens epithelial cells (HLECs) from patients older than 60 years. Ki67+, Sox2+, and Abcg2+ cells were not observed in the cultured primary HLECs from patients older than 60 years, representative pictures showed as A–B. Scale bar, 40 μm.

Mentions: In some tissues, adult stem cells, which spend most of their time in quiescent state, are activated by stimulations such as injury and start to express different markers.[18] In the present study, whether the LSCs from older patients temporarily lost the expression of stem cell markers and would regain their properties in certain circumstances, such as after cataract surgery, was examined. The expressions of Sox2, Ki67, and Abcg2 were examined in primary HLECs from individuals older than 60 years as soon as the HLECs migrated from the explants. Unsurprizingly, no signal was detected for Sox2, Ki67, and Abcg2 in cultured primary HLECs (Fig. 2), thus suggesting that properties of LSCs were genuinely lost and could not be retrieved after stimulation.


Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans
Expressions of Ki67, Sox2, and Abcg2 in the cultured primary human lens epithelial cells (HLECs) from patients older than 60 years. Ki67+, Sox2+, and Abcg2+ cells were not observed in the cultured primary HLECs from patients older than 60 years, representative pictures showed as A–B. Scale bar, 40 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998311&req=5

Figure 2: Expressions of Ki67, Sox2, and Abcg2 in the cultured primary human lens epithelial cells (HLECs) from patients older than 60 years. Ki67+, Sox2+, and Abcg2+ cells were not observed in the cultured primary HLECs from patients older than 60 years, representative pictures showed as A–B. Scale bar, 40 μm.
Mentions: In some tissues, adult stem cells, which spend most of their time in quiescent state, are activated by stimulations such as injury and start to express different markers.[18] In the present study, whether the LSCs from older patients temporarily lost the expression of stem cell markers and would regain their properties in certain circumstances, such as after cataract surgery, was examined. The expressions of Sox2, Ki67, and Abcg2 were examined in primary HLECs from individuals older than 60 years as soon as the HLECs migrated from the explants. Unsurprizingly, no signal was detected for Sox2, Ki67, and Abcg2 in cultured primary HLECs (Fig. 2), thus suggesting that properties of LSCs were genuinely lost and could not be retrieved after stimulation.

View Article: PubMed Central - PubMed

ABSTRACT

The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.

Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50–60, 60–80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-β-gal+ HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-β-gal+) HLECs and the severity of ARCs were analyzed.

Ki67+, Sox2+, and Abcg2+ HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-β-gal+ primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-β-gal+ and senescent HLECs.

Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.

No MeSH data available.