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Characterization of Mycobacterium Abscessus Subtypes in Shanghai of China

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the study was to investigate the epidemic characteristics of Mycobacterium abscessus in Shanghai.

Fifty-five strains from 55 M. abscessus pulmonary disease patients were isolated. Drug sensitivity was measured by a broth microdilution method. Subtypes of M. abscessus were identified by DNA sequencing. Multilocus sequence typing (MLST), mining spanning tree (MST), and pulsed-field gel electrophoresis (PFGE) were used to analyze sequence types (ST) and clonal complexes (CC). Clinical manifestations were assessed by CT imaging.

We identified 42 A isolates, 11 M, and 2 B-subtypes. A and M were highly sensitive to tigecycline and amikacin (97.6–100%). The A-type easily developed drug resistance against clarithromycin. Both types were highly resistance to sulfonamides, moxifloxacin, doxycycline, imipenem, and tobramycin. MLST analysis identified 41 STs including 32 new STs. The MST algorithm distributed 55 isolates into 12 separate CC. The PFGE analysis exhibited 53 distinct restriction patterns and the M-type was closely clustered according to their ST and CC numbers. CT imaging showed that tree-in-bud and patch shadow were commonly observed in M-type, whereas pulmonary cavities were often found in A-type infection patients (P < 0.001).

ST1 in A and ST23 in M-type were the main epidemic strains in Shanghai. The M-type appeared to be prone to epidemic nosocomial transmission.

No MeSH data available.


Distribution of sequence types (STs) in 55 isolates by multilocus sequence typing (MLST). MLST = multilocus sequence typing, ST = sequence types.
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Figure 1: Distribution of sequence types (STs) in 55 isolates by multilocus sequence typing (MLST). MLST = multilocus sequence typing, ST = sequence types.

Mentions: A comparative analysis utilizing references in the website www.pasteur.fr/mlst found neither base loss nor insertion in all the 7 housekeeping genes examined. Additionally, a new gene, ArgH34, was identified. Analysis of the 55 isolates demonstrated that the sequence types ST1 in the A-type and ST 23 in the M-type were in the majority and that 32 new STs were identified. The data was submitted to the allele distribution inquiry website and new numbers ST179-ST211 were assigned. Bacteria isolates were subjected to the aggregation analysis by the NJ method together with reference strains (ATCC19977 for A-type, CIP18297 for M-type, and CIP108541 for B-type) and categorized into 3 groups; 42 strains in A-type with 33 STs, 2 in B-type with 2 STs, and 11 in M-type with 6 STs (Figure 1). The result was consistent with the sequencing analysis of the rpoB, erm(41), and PRA-hsp65 genes.


Characterization of Mycobacterium Abscessus Subtypes in Shanghai of China
Distribution of sequence types (STs) in 55 isolates by multilocus sequence typing (MLST). MLST = multilocus sequence typing, ST = sequence types.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998240&req=5

Figure 1: Distribution of sequence types (STs) in 55 isolates by multilocus sequence typing (MLST). MLST = multilocus sequence typing, ST = sequence types.
Mentions: A comparative analysis utilizing references in the website www.pasteur.fr/mlst found neither base loss nor insertion in all the 7 housekeeping genes examined. Additionally, a new gene, ArgH34, was identified. Analysis of the 55 isolates demonstrated that the sequence types ST1 in the A-type and ST 23 in the M-type were in the majority and that 32 new STs were identified. The data was submitted to the allele distribution inquiry website and new numbers ST179-ST211 were assigned. Bacteria isolates were subjected to the aggregation analysis by the NJ method together with reference strains (ATCC19977 for A-type, CIP18297 for M-type, and CIP108541 for B-type) and categorized into 3 groups; 42 strains in A-type with 33 STs, 2 in B-type with 2 STs, and 11 in M-type with 6 STs (Figure 1). The result was consistent with the sequencing analysis of the rpoB, erm(41), and PRA-hsp65 genes.

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the study was to investigate the epidemic characteristics of Mycobacterium abscessus in Shanghai.

Fifty-five strains from 55 M. abscessus pulmonary disease patients were isolated. Drug sensitivity was measured by a broth microdilution method. Subtypes of M. abscessus were identified by DNA sequencing. Multilocus sequence typing (MLST), mining spanning tree (MST), and pulsed-field gel electrophoresis (PFGE) were used to analyze sequence types (ST) and clonal complexes (CC). Clinical manifestations were assessed by CT imaging.

We identified 42 A isolates, 11 M, and 2 B-subtypes. A and M were highly sensitive to tigecycline and amikacin (97.6–100%). The A-type easily developed drug resistance against clarithromycin. Both types were highly resistance to sulfonamides, moxifloxacin, doxycycline, imipenem, and tobramycin. MLST analysis identified 41 STs including 32 new STs. The MST algorithm distributed 55 isolates into 12 separate CC. The PFGE analysis exhibited 53 distinct restriction patterns and the M-type was closely clustered according to their ST and CC numbers. CT imaging showed that tree-in-bud and patch shadow were commonly observed in M-type, whereas pulmonary cavities were often found in A-type infection patients (P < 0.001).

ST1 in A and ST23 in M-type were the main epidemic strains in Shanghai. The M-type appeared to be prone to epidemic nosocomial transmission.

No MeSH data available.