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Protective effect of Shenfu injection preconditioning on lung ischemia-reperfusion injury

View Article: PubMed Central - PubMed

ABSTRACT

Lung ischemia-reperfusion injury remains a problem in thoracic surgery, as minimal progress has been made concerning its prevention and control. In the present study, the protective effects and the underlying mechanism of Shenfu injection preconditioning on a rat lung ischemia-reperfusion model was investigated. Shenfu injection is a well-known Chinese traditional medicine, which is composed of Red Radix Ginseng and Radix Aconitum carmichaelii, with ginseng saponin and aconitum alkaloids as the active ingredients. A total of 72 specific pathogen-free, healthy male Wistar rats were randomly divided into control, model and Shenfu injection (10 ml/kg injection prior to injury) groups and were assessed at the following points: Ischemia 45 min; reperfusion 60 min; and reperfusion 120 min. Blood collected from the aorta abdominalis was cryopreserved at −70°C for the analysis of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. Lung tissues were divided into three equal sections in order to assess the wet-to-dry (W/D) lung ratio, tumor necrosis factor (TNF)-α expression levels, myeloperoxidase (MPO) activity, alveolar damage, total protein and hematoxylin and eosin staining. The results demonstrated that the lung W/D weight ratio, TNF-α expression levels and SOD activity in the Shenfu group were significantly lower at 120 min reperfusion (P<0.05), as compared with the model group. MPO and MDA activity significantly decreased following reperfusion at 60 and 120 min (P<0.05), as compared with the model group. In addition, the degree of alveolar damage in the Shenfu group was significantly decreased (P<0.05), as compared with the model group. In addition, compared with the model group, the degree of alveolar damage in the Shenfu group was significantly lower (P<0.05); however, no significant changes in total protein were observed. The extent of alveolar structural damage and the proportion of interstitial neutrophils and alveolar and interstitial red blood cells were lower in the Shenfu group, as compared with the model and control groups. Therefore, the results of the present study suggested that Shenfu injection may have protective effects on lung ischemia-reperfusion injury.

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Hematoxylin and eosin staining of (A) the control, (B) model and (C) Shenfu groups. No signs of alveolar damage were detected in the control group; whereas the model group exhibited severely damaged alveolar structures, serous effusion, a large quantity of red blood cells and neutrophil infiltration. The extent of the damage to the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was lower in the Shenfu group compared with the control and model groups.
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f7-etm-0-0-3549: Hematoxylin and eosin staining of (A) the control, (B) model and (C) Shenfu groups. No signs of alveolar damage were detected in the control group; whereas the model group exhibited severely damaged alveolar structures, serous effusion, a large quantity of red blood cells and neutrophil infiltration. The extent of the damage to the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was lower in the Shenfu group compared with the control and model groups.

Mentions: The control group exhibited no signs of alveolar damage, with visible organization of alveolar wall integrity, and no interstitial lung edema and neutrophil infiltration. The model group exhibited severely damaged alveolar structures, a large quantity of alveoli, congestive alveolar walls, severe pulmonary interval thickening, wide alveolar spaces, interstitial edema, serous effusion, a large quantity of red blood cells and neutrophil infiltration. Notably, the extent of the damage in the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was reduced in the Shenfu group, as compared with the control and model groups (Fig. 7). The results indicated that Shenfu injection may relieve the degree of alveolar structure damage.


Protective effect of Shenfu injection preconditioning on lung ischemia-reperfusion injury
Hematoxylin and eosin staining of (A) the control, (B) model and (C) Shenfu groups. No signs of alveolar damage were detected in the control group; whereas the model group exhibited severely damaged alveolar structures, serous effusion, a large quantity of red blood cells and neutrophil infiltration. The extent of the damage to the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was lower in the Shenfu group compared with the control and model groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998227&req=5

f7-etm-0-0-3549: Hematoxylin and eosin staining of (A) the control, (B) model and (C) Shenfu groups. No signs of alveolar damage were detected in the control group; whereas the model group exhibited severely damaged alveolar structures, serous effusion, a large quantity of red blood cells and neutrophil infiltration. The extent of the damage to the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was lower in the Shenfu group compared with the control and model groups.
Mentions: The control group exhibited no signs of alveolar damage, with visible organization of alveolar wall integrity, and no interstitial lung edema and neutrophil infiltration. The model group exhibited severely damaged alveolar structures, a large quantity of alveoli, congestive alveolar walls, severe pulmonary interval thickening, wide alveolar spaces, interstitial edema, serous effusion, a large quantity of red blood cells and neutrophil infiltration. Notably, the extent of the damage in the alveolar structure, interstitial neutrophils, and alveolar and interstitial red blood cells was reduced in the Shenfu group, as compared with the control and model groups (Fig. 7). The results indicated that Shenfu injection may relieve the degree of alveolar structure damage.

View Article: PubMed Central - PubMed

ABSTRACT

Lung ischemia-reperfusion injury remains a problem in thoracic surgery, as minimal progress has been made concerning its prevention and control. In the present study, the protective effects and the underlying mechanism of Shenfu injection preconditioning on a rat lung ischemia-reperfusion model was investigated. Shenfu injection is a well-known Chinese traditional medicine, which is composed of Red Radix Ginseng and Radix Aconitum carmichaelii, with ginseng saponin and aconitum alkaloids as the active ingredients. A total of 72 specific pathogen-free, healthy male Wistar rats were randomly divided into control, model and Shenfu injection (10 ml/kg injection prior to injury) groups and were assessed at the following points: Ischemia 45 min; reperfusion 60 min; and reperfusion 120 min. Blood collected from the aorta abdominalis was cryopreserved at −70°C for the analysis of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. Lung tissues were divided into three equal sections in order to assess the wet-to-dry (W/D) lung ratio, tumor necrosis factor (TNF)-α expression levels, myeloperoxidase (MPO) activity, alveolar damage, total protein and hematoxylin and eosin staining. The results demonstrated that the lung W/D weight ratio, TNF-α expression levels and SOD activity in the Shenfu group were significantly lower at 120 min reperfusion (P<0.05), as compared with the model group. MPO and MDA activity significantly decreased following reperfusion at 60 and 120 min (P<0.05), as compared with the model group. In addition, the degree of alveolar damage in the Shenfu group was significantly decreased (P<0.05), as compared with the model group. In addition, compared with the model group, the degree of alveolar damage in the Shenfu group was significantly lower (P<0.05); however, no significant changes in total protein were observed. The extent of alveolar structural damage and the proportion of interstitial neutrophils and alveolar and interstitial red blood cells were lower in the Shenfu group, as compared with the model and control groups. Therefore, the results of the present study suggested that Shenfu injection may have protective effects on lung ischemia-reperfusion injury.

No MeSH data available.


Related in: MedlinePlus