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Protective effect of Shenfu injection preconditioning on lung ischemia-reperfusion injury

View Article: PubMed Central - PubMed

ABSTRACT

Lung ischemia-reperfusion injury remains a problem in thoracic surgery, as minimal progress has been made concerning its prevention and control. In the present study, the protective effects and the underlying mechanism of Shenfu injection preconditioning on a rat lung ischemia-reperfusion model was investigated. Shenfu injection is a well-known Chinese traditional medicine, which is composed of Red Radix Ginseng and Radix Aconitum carmichaelii, with ginseng saponin and aconitum alkaloids as the active ingredients. A total of 72 specific pathogen-free, healthy male Wistar rats were randomly divided into control, model and Shenfu injection (10 ml/kg injection prior to injury) groups and were assessed at the following points: Ischemia 45 min; reperfusion 60 min; and reperfusion 120 min. Blood collected from the aorta abdominalis was cryopreserved at −70°C for the analysis of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. Lung tissues were divided into three equal sections in order to assess the wet-to-dry (W/D) lung ratio, tumor necrosis factor (TNF)-α expression levels, myeloperoxidase (MPO) activity, alveolar damage, total protein and hematoxylin and eosin staining. The results demonstrated that the lung W/D weight ratio, TNF-α expression levels and SOD activity in the Shenfu group were significantly lower at 120 min reperfusion (P<0.05), as compared with the model group. MPO and MDA activity significantly decreased following reperfusion at 60 and 120 min (P<0.05), as compared with the model group. In addition, the degree of alveolar damage in the Shenfu group was significantly decreased (P<0.05), as compared with the model group. In addition, compared with the model group, the degree of alveolar damage in the Shenfu group was significantly lower (P<0.05); however, no significant changes in total protein were observed. The extent of alveolar structural damage and the proportion of interstitial neutrophils and alveolar and interstitial red blood cells were lower in the Shenfu group, as compared with the model and control groups. Therefore, the results of the present study suggested that Shenfu injection may have protective effects on lung ischemia-reperfusion injury.

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Lung W/D ratio among the groups at three time points. *P<0.05 vs. the control; **P<0.05 vs. the ischemia time point. W/D, weight/dry weight.
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f1-etm-0-0-3549: Lung W/D ratio among the groups at three time points. *P<0.05 vs. the control; **P<0.05 vs. the ischemia time point. W/D, weight/dry weight.

Mentions: The lung W/D ratio was not significantly different at the three time points in the control group, whereas the lung W/D ratios of rats in the model and Shenfu groups significantly increased with prolonged reperfusion (P<0.05). The model and Shenfu groups were not significantly different at the ischemia 45 min time point, as compared with the control group, but were significantly increased at the reperfusion 60 min and reperfusion 120 min time points (P<0.05). Lung W/D ratios in the Shenfu group were decreased at reperfusion 60 min and 120 min, compared with the model group, however, there were no significant differences (P>0.05). Thus the results revealed that Shenfu injection can reduce the W/D ratios at reperfusion times (Fig. 1).


Protective effect of Shenfu injection preconditioning on lung ischemia-reperfusion injury
Lung W/D ratio among the groups at three time points. *P<0.05 vs. the control; **P<0.05 vs. the ischemia time point. W/D, weight/dry weight.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998227&req=5

f1-etm-0-0-3549: Lung W/D ratio among the groups at three time points. *P<0.05 vs. the control; **P<0.05 vs. the ischemia time point. W/D, weight/dry weight.
Mentions: The lung W/D ratio was not significantly different at the three time points in the control group, whereas the lung W/D ratios of rats in the model and Shenfu groups significantly increased with prolonged reperfusion (P<0.05). The model and Shenfu groups were not significantly different at the ischemia 45 min time point, as compared with the control group, but were significantly increased at the reperfusion 60 min and reperfusion 120 min time points (P<0.05). Lung W/D ratios in the Shenfu group were decreased at reperfusion 60 min and 120 min, compared with the model group, however, there were no significant differences (P>0.05). Thus the results revealed that Shenfu injection can reduce the W/D ratios at reperfusion times (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

Lung ischemia-reperfusion injury remains a problem in thoracic surgery, as minimal progress has been made concerning its prevention and control. In the present study, the protective effects and the underlying mechanism of Shenfu injection preconditioning on a rat lung ischemia-reperfusion model was investigated. Shenfu injection is a well-known Chinese traditional medicine, which is composed of Red Radix Ginseng and Radix Aconitum carmichaelii, with ginseng saponin and aconitum alkaloids as the active ingredients. A total of 72 specific pathogen-free, healthy male Wistar rats were randomly divided into control, model and Shenfu injection (10 ml/kg injection prior to injury) groups and were assessed at the following points: Ischemia 45 min; reperfusion 60 min; and reperfusion 120 min. Blood collected from the aorta abdominalis was cryopreserved at &minus;70&deg;C for the analysis of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. Lung tissues were divided into three equal sections in order to assess the wet-to-dry (W/D) lung ratio, tumor necrosis factor (TNF)-&alpha; expression levels, myeloperoxidase (MPO) activity, alveolar damage, total protein and hematoxylin and eosin staining. The results demonstrated that the lung W/D weight ratio, TNF-&alpha; expression levels and SOD activity in the Shenfu group were significantly lower at 120 min reperfusion (P&lt;0.05), as compared with the model group. MPO and MDA activity significantly decreased following reperfusion at 60 and 120 min (P&lt;0.05), as compared with the model group. In addition, the degree of alveolar damage in the Shenfu group was significantly decreased (P&lt;0.05), as compared with the model group. In addition, compared with the model group, the degree of alveolar damage in the Shenfu group was significantly lower (P&lt;0.05); however, no significant changes in total protein were observed. The extent of alveolar structural damage and the proportion of interstitial neutrophils and alveolar and interstitial red blood cells were lower in the Shenfu group, as compared with the model and control groups. Therefore, the results of the present study suggested that Shenfu injection may have protective effects on lung ischemia-reperfusion injury.

No MeSH data available.


Related in: MedlinePlus