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Expression of microphthalmia transcription factor, S100 protein, and HMB-45 in malignant melanoma and pigmented nevi

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ABSTRACT

Malignant melanoma (MM) is a type of malignant tumor, which originates from neural crest melanocytes. MM progresses rapidly and results in a high mortality rate. The present study aims to investigate the expression of microphthalmia transcription factor (MITF), the S100 protein, and HMB-45 in MM and pigmented nevi. A total of 32 MM samples (including three skin metastasis, three lymph node metastasis and two spindle cell MM samples), two Spitz nevus samples, four pigmented nevus samples and two blue nevus samples were collected. The expression levels of S100 protein, HMB-45, and MITF were observed via immunostaining. The S100 protein exhibited high positive rates in MM and pigment disorders (96.7 and 100%, respectively), but with low specificity. The S100 protein was also expressed in fibroblasts, myoepithelial cells, histocytes and Langerhans cells in normal skin samples. HMB-45 had high specificity. Its positive expression was only confined to MM cells and junctional nevus cells. Furthermore, HMB-45 was not expressed in melanocytes in the normal tissue samples around the tumor or in the benign intradermal nevus cells. MITF exhibited high specificity and high sensitivity. It was expressed in the nuclei of melanocytes, MM cells and nevus cells. It was observed to be strongly expressed in metastatic MM and spindle cell MMs. Thus, MITF may present as a specific immunomarker for the diagnosis and differential diagnosis of MM.

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HMB-45 expression in normal Langerhans cells without skin damage caused by melanoma (magnification, ×400).
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f4-br-0-0-732: HMB-45 expression in normal Langerhans cells without skin damage caused by melanoma (magnification, ×400).

Mentions: S100 immunostaining demonstrated that the positive rates of S100 protein in MM and pigmented nevi were 96.8 and 100%, respectively (Table I and Fig. 1). Langerhans cells, musculoepithelial cells, and a small number of fibrocytes in normal tissue samples also exhibited S100 positivity (Fig. 2). In addition, the four pigmented nevus samples, two blue nevus samples and two Spitz nevus samples exhibited S100-positive expression (Fig. 3). HMB-45 immunostaining indicated that the positive rate of HMB-45 in MM was 81.2%. Positive HMB-45 expression was only confined to MM, active pigmented nevus cells and Spitz nevus cells, and was not expressed in the normal Langerhans cells (Fig. 4). MITF immunostaining showed that the positive rates of MITF expression in MM and pigmented nevus cells were 96.8 and 100%, respectively. Positive expression of MITF was not observed in the components of skin tissue samples, apart from in melanocytes, nevocytes and in MM cells.


Expression of microphthalmia transcription factor, S100 protein, and HMB-45 in malignant melanoma and pigmented nevi
HMB-45 expression in normal Langerhans cells without skin damage caused by melanoma (magnification, ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998223&req=5

f4-br-0-0-732: HMB-45 expression in normal Langerhans cells without skin damage caused by melanoma (magnification, ×400).
Mentions: S100 immunostaining demonstrated that the positive rates of S100 protein in MM and pigmented nevi were 96.8 and 100%, respectively (Table I and Fig. 1). Langerhans cells, musculoepithelial cells, and a small number of fibrocytes in normal tissue samples also exhibited S100 positivity (Fig. 2). In addition, the four pigmented nevus samples, two blue nevus samples and two Spitz nevus samples exhibited S100-positive expression (Fig. 3). HMB-45 immunostaining indicated that the positive rate of HMB-45 in MM was 81.2%. Positive HMB-45 expression was only confined to MM, active pigmented nevus cells and Spitz nevus cells, and was not expressed in the normal Langerhans cells (Fig. 4). MITF immunostaining showed that the positive rates of MITF expression in MM and pigmented nevus cells were 96.8 and 100%, respectively. Positive expression of MITF was not observed in the components of skin tissue samples, apart from in melanocytes, nevocytes and in MM cells.

View Article: PubMed Central - PubMed

ABSTRACT

Malignant melanoma (MM) is a type of malignant tumor, which originates from neural crest melanocytes. MM progresses rapidly and results in a high mortality rate. The present study aims to investigate the expression of microphthalmia transcription factor (MITF), the S100 protein, and HMB-45 in MM and pigmented nevi. A total of 32 MM samples (including three skin metastasis, three lymph node metastasis and two spindle cell MM samples), two Spitz nevus samples, four pigmented nevus samples and two blue nevus samples were collected. The expression levels of S100 protein, HMB-45, and MITF were observed via immunostaining. The S100 protein exhibited high positive rates in MM and pigment disorders (96.7 and 100%, respectively), but with low specificity. The S100 protein was also expressed in fibroblasts, myoepithelial cells, histocytes and Langerhans cells in normal skin samples. HMB-45 had high specificity. Its positive expression was only confined to MM cells and junctional nevus cells. Furthermore, HMB-45 was not expressed in melanocytes in the normal tissue samples around the tumor or in the benign intradermal nevus cells. MITF exhibited high specificity and high sensitivity. It was expressed in the nuclei of melanocytes, MM cells and nevus cells. It was observed to be strongly expressed in metastatic MM and spindle cell MMs. Thus, MITF may present as a specific immunomarker for the diagnosis and differential diagnosis of MM.

No MeSH data available.


Related in: MedlinePlus