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MiR-10a improves hepatic fibrosis by regulating the TGF β l/Smads signal transduction pathway

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to examine the expression variation of the mouse hepatic fibrosis tissue transforming growth factor (TGF)-βl/Smads signal transduction pathway and its correlation with progression of hepatic fibrosis. The promotion effect of microRNA (miR)-10a on hepatic fibrosis and its possible mechanism was also assessed. Forty healthy female 8-week-old C57BL6/J mice were randomly divided into the control group (intraperitoneal injection of 5 µl/g normal saline, twice per week for 8 weeks) and the hepatic fibrosis group (intraperitoneal injection of 5 µl/g 10% CCI4 olive oil, twice per week for 8 weeks), with 20 mice per group. RT-PCR was used to test miR-10a expression in cells in the control and hepatic fibrosis groups. Cell culture and transfection of miR-10a mimics were conducted in the two groups and a Cell Counting Kit-8 was used to test the expression of TGF-β1 and Smad7 in hepatic fibroblasts. It was found that in comparison with the control group, miR-10a expression was significantly increased in the hepatic fibrosis group compared with the control group (P<0.05). The expression quantity of miR-10a was significantly increased in the transfection group compared with the control group (P<0.05). A high expression of miR-10a significantly improved TGF-β1 expression and reduced Smad7 expression in the hepatic fibrosis group (P<0.05). In conclusion, miR-10a expression was high in mouse hepatic tissues, transfection of miR-10a mimics significantly promoted the cell proliferation of hepatic fibrosis, and miR-10a improved hepatic fibrosis by regulating the TGF-βl/Smads signal transduction pathway.

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Related in: MedlinePlus

MicroRNA (miR)-10a was low-expressed in hepatic fibrosis cell transfected with miR-10a mimics (**compared with control, P<0.05).
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f1-etm-0-0-3542: MicroRNA (miR)-10a was low-expressed in hepatic fibrosis cell transfected with miR-10a mimics (**compared with control, P<0.05).

Mentions: miR-10a expression quantity in cells in the transfection group was approximately 1/16 of that in the control group (Fig. 1).


MiR-10a improves hepatic fibrosis by regulating the TGF β l/Smads signal transduction pathway
MicroRNA (miR)-10a was low-expressed in hepatic fibrosis cell transfected with miR-10a mimics (**compared with control, P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998216&req=5

f1-etm-0-0-3542: MicroRNA (miR)-10a was low-expressed in hepatic fibrosis cell transfected with miR-10a mimics (**compared with control, P<0.05).
Mentions: miR-10a expression quantity in cells in the transfection group was approximately 1/16 of that in the control group (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to examine the expression variation of the mouse hepatic fibrosis tissue transforming growth factor (TGF)-&beta;l/Smads signal transduction pathway and its correlation with progression of hepatic fibrosis. The promotion effect of microRNA (miR)-10a on hepatic fibrosis and its possible mechanism was also assessed. Forty healthy female 8-week-old C57BL6/J mice were randomly divided into the control group (intraperitoneal injection of 5 &micro;l/g normal saline, twice per week for 8 weeks) and the hepatic fibrosis group (intraperitoneal injection of 5 &micro;l/g 10% CCI4 olive oil, twice per week for 8 weeks), with 20 mice per group. RT-PCR was used to test miR-10a expression in cells in the control and hepatic fibrosis groups. Cell culture and transfection of miR-10a mimics were conducted in the two groups and a Cell Counting Kit-8 was used to test the expression of TGF-&beta;1 and Smad7 in hepatic fibroblasts. It was found that in comparison with the control group, miR-10a expression was significantly increased in the hepatic fibrosis group compared with the control group (P&lt;0.05). The expression quantity of miR-10a was significantly increased in the transfection group compared with the control group (P&lt;0.05). A high expression of miR-10a significantly improved TGF-&beta;1 expression and reduced Smad7 expression in the hepatic fibrosis group (P&lt;0.05). In conclusion, miR-10a expression was high in mouse hepatic tissues, transfection of miR-10a mimics significantly promoted the cell proliferation of hepatic fibrosis, and miR-10a improved hepatic fibrosis by regulating the TGF-&beta;l/Smads signal transduction pathway.

No MeSH data available.


Related in: MedlinePlus