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Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the study was to investigate the possibility of human umbilical cord mesenchymal stem cells (UC-MSCs) surviving and differentiating into hepatocyte-like cells in partially hepatectomized model rats. MSCs were isolated from human umbilical cord and cultured with collagenase digestion. Cell surface markers were detected and fifth generation UC-MSCs were labeled with PKH26. The partially hepatectomized model rats were injected with the labeled human umbilical cord MSCs and transplanted through the portal vein. The survival of the labeled cells, in differentiation conditions and the expression of hepatic marker albumin were observed at post-transplantation 1, 2 and 3 weeks under a fluorescence microscope. It was found that the human umbilical cord MSCs could be cultured and amplified in vitro. Following transplantation to the partially hepatectomized liver of the model rat, the cells survived and expresses the hepatic marker albumin in vivo. After being labeled with PKH26, the cells were visualized as red fluorescence under a fluorescence microscope. In the frozen sections of the liver, the marked cells scattered around and most of them expressed albumin with green fluorescence under the fluorescence microscope. In conclusion, the transplanted human umbilical cord MSCs survived and differentiated into hepatocyte-like cells. The human umbilical cord MSCs may therefore be a main source of hepatocytes in transplantation.

No MeSH data available.


(A) Expression of albumin in MSCs (×100) in the first week; (B) the expression of albumin in MSCs (×100) in the second week; (5) the expression of albumin in MSCs (×100) in the third week. MSCs, mesenchymal stem cells.
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f5-etm-0-0-3543: (A) Expression of albumin in MSCs (×100) in the first week; (B) the expression of albumin in MSCs (×100) in the second week; (5) the expression of albumin in MSCs (×100) in the third week. MSCs, mesenchymal stem cells.

Mentions: Rats were sacrificed in the first, second, and third week, after which the abdominal cavity was opened to observe the liver. There were clear and broad adhesions between the liver and surrounding tissues, the surface of the liver was uneven, and at the ligature where part of the liver was cut, the tissue was firm or hard. The rat liver was dissected to make frozen sections and the position of the labeled cells in liver was observed under the fluorescence microscope. The tagged red fluorescence cells were scattered in the liver and some were embedded in the liver panel (Fig. 4A-C). Due to cell differentiation, the red fluorescence gradually faded and after immunofluorescence staining, labeled cells with albumin staining were detected as positive, and excited green fluorescence (Fig. 5A-C), indicating that the human umbilical cord produces white protein. In addition, after H&E staining there were a large number of cells aggregating around the hepatic sinusoid, and hyperplasia was relatively active (Fig. 6A and B).


Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats
(A) Expression of albumin in MSCs (×100) in the first week; (B) the expression of albumin in MSCs (×100) in the second week; (5) the expression of albumin in MSCs (×100) in the third week. MSCs, mesenchymal stem cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998204&req=5

f5-etm-0-0-3543: (A) Expression of albumin in MSCs (×100) in the first week; (B) the expression of albumin in MSCs (×100) in the second week; (5) the expression of albumin in MSCs (×100) in the third week. MSCs, mesenchymal stem cells.
Mentions: Rats were sacrificed in the first, second, and third week, after which the abdominal cavity was opened to observe the liver. There were clear and broad adhesions between the liver and surrounding tissues, the surface of the liver was uneven, and at the ligature where part of the liver was cut, the tissue was firm or hard. The rat liver was dissected to make frozen sections and the position of the labeled cells in liver was observed under the fluorescence microscope. The tagged red fluorescence cells were scattered in the liver and some were embedded in the liver panel (Fig. 4A-C). Due to cell differentiation, the red fluorescence gradually faded and after immunofluorescence staining, labeled cells with albumin staining were detected as positive, and excited green fluorescence (Fig. 5A-C), indicating that the human umbilical cord produces white protein. In addition, after H&E staining there were a large number of cells aggregating around the hepatic sinusoid, and hyperplasia was relatively active (Fig. 6A and B).

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the study was to investigate the possibility of human umbilical cord mesenchymal stem cells (UC-MSCs) surviving and differentiating into hepatocyte-like cells in partially hepatectomized model rats. MSCs were isolated from human umbilical cord and cultured with collagenase digestion. Cell surface markers were detected and fifth generation UC-MSCs were labeled with PKH26. The partially hepatectomized model rats were injected with the labeled human umbilical cord MSCs and transplanted through the portal vein. The survival of the labeled cells, in differentiation conditions and the expression of hepatic marker albumin were observed at post-transplantation 1, 2 and 3 weeks under a fluorescence microscope. It was found that the human umbilical cord MSCs could be cultured and amplified in vitro. Following transplantation to the partially hepatectomized liver of the model rat, the cells survived and expresses the hepatic marker albumin in vivo. After being labeled with PKH26, the cells were visualized as red fluorescence under a fluorescence microscope. In the frozen sections of the liver, the marked cells scattered around and most of them expressed albumin with green fluorescence under the fluorescence microscope. In conclusion, the transplanted human umbilical cord MSCs survived and differentiated into hepatocyte-like cells. The human umbilical cord MSCs may therefore be a main source of hepatocytes in transplantation.

No MeSH data available.