Limits...
Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

View Article: PubMed Central - PubMed

ABSTRACT

Oral cavity squamous cell carcinoma may be more aggressive at presentation and recurrence in young patients compared with older patients. Dysregulation of microRNAs (miRNAs or miRs) has been associated with the development and prognosis of oral cavity cancer. The present study investigated miRNA expression in carcinoma of the oral tongue in young patients. miRNA expression profiles were evaluated in formalin-fixed, paraffin-embedded samples of tumor and normal mucosa from 12 patients aged <30 years old with squamous cell carcinoma of the tongue. The levels of let-7f-5p, miR-30b-5p and let-7e-5p were upregulated in tumors (P<0.05). The expression of let-7f-5p was upregulated in non-aggressive tumors, while the expression of let-7e-5p was upregulated in aggressive tumors, compared with the corresponding normal tissue. Aggressive tumors had higher levels of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p than non-aggressive tumors (P<0.05). The findings remained significant for let-7c upon false-discovery rate correction. An excellent correlation was noticed on validation of NanoString counts by quantitative polymerase chain reaction. The comparison with published findings in adults demonstrated a unique miRNA signature in young patients with aggressive disease. Aggressive oral cavity cancer in patients <30 years old is associated with a distinctive expression pattern of the let-7 family. Larger studies including direct comparison with older patients are warranted.

No MeSH data available.


Related in: MedlinePlus

Heatmap of differentially expressed microRNAs (Z-scores of log2 values) in aggressive and non-aggressive tumors. Red indicates high expression and blue low expression. miR/miRNA, microRNA; hsa, Homo sapiens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4998201&req=5

f1-ol-0-0-4892: Heatmap of differentially expressed microRNAs (Z-scores of log2 values) in aggressive and non-aggressive tumors. Red indicates high expression and blue low expression. miR/miRNA, microRNA; hsa, Homo sapiens.

Mentions: Fig. 1 represents the differential expression of the miRNAs with the highest expression levels, while Fig. 2 represents the expression of the significantly altered miRNAs in aggressive vs. non-aggressive tumors. There was a significant upregulation of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p in the aggressive tumors (P<0.05); the difference for let-7c remained significant after FDR correction. Additionally, the aggressive tumors exhibited downregulation of miR-181a-5p and let-7i-5p (P=0.06).


Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients
Heatmap of differentially expressed microRNAs (Z-scores of log2 values) in aggressive and non-aggressive tumors. Red indicates high expression and blue low expression. miR/miRNA, microRNA; hsa, Homo sapiens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998201&req=5

f1-ol-0-0-4892: Heatmap of differentially expressed microRNAs (Z-scores of log2 values) in aggressive and non-aggressive tumors. Red indicates high expression and blue low expression. miR/miRNA, microRNA; hsa, Homo sapiens.
Mentions: Fig. 1 represents the differential expression of the miRNAs with the highest expression levels, while Fig. 2 represents the expression of the significantly altered miRNAs in aggressive vs. non-aggressive tumors. There was a significant upregulation of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p in the aggressive tumors (P<0.05); the difference for let-7c remained significant after FDR correction. Additionally, the aggressive tumors exhibited downregulation of miR-181a-5p and let-7i-5p (P=0.06).

View Article: PubMed Central - PubMed

ABSTRACT

Oral cavity squamous cell carcinoma may be more aggressive at presentation and recurrence in young patients compared with older patients. Dysregulation of microRNAs (miRNAs or miRs) has been associated with the development and prognosis of oral cavity cancer. The present study investigated miRNA expression in carcinoma of the oral tongue in young patients. miRNA expression profiles were evaluated in formalin-fixed, paraffin-embedded samples of tumor and normal mucosa from 12 patients aged &lt;30 years old with squamous cell carcinoma of the tongue. The levels of let-7f-5p, miR-30b-5p and let-7e-5p were upregulated in tumors (P&lt;0.05). The expression of let-7f-5p was upregulated in non-aggressive tumors, while the expression of let-7e-5p was upregulated in aggressive tumors, compared with the corresponding normal tissue. Aggressive tumors had higher levels of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p than non-aggressive tumors (P&lt;0.05). The findings remained significant for let-7c upon false-discovery rate correction. An excellent correlation was noticed on validation of NanoString counts by quantitative polymerase chain reaction. The comparison with published findings in adults demonstrated a unique miRNA signature in young patients with aggressive disease. Aggressive oral cavity cancer in patients &lt;30 years old is associated with a distinctive expression pattern of the let-7 family. Larger studies including direct comparison with older patients are warranted.

No MeSH data available.


Related in: MedlinePlus