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Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

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ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.


The expression of protein in MCF-7 cells treated with T. chinensis flavonoids detected using western blot analysis. 1, control group; 2, 0.0991 mg/ml; 3, 0.1982 mg/ml; 4, 0.3964 mg/ml; 5,0.7928 mg/ml; 6, 1.5856 mg/ml.
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f6-ol-0-0-4898: The expression of protein in MCF-7 cells treated with T. chinensis flavonoids detected using western blot analysis. 1, control group; 2, 0.0991 mg/ml; 3, 0.1982 mg/ml; 4, 0.3964 mg/ml; 5,0.7928 mg/ml; 6, 1.5856 mg/ml.

Mentions: In order to study the mechanism of flavonoid-induced apoptosis in MCF-7 cells, the expression of caspases, fasL, p53 and bcl-2 were determined. After 24 h of exposure to flavonoids, the expression of cleaved caspase-3 and caspase-9 increased in a dose-dependent manner. Bcl-2 is a crucial determinant of cell apoptosis, and a decrease in its expression was observed in the flavonoid-treated cells. This observation suggests that an intrinsic apoptotic signaling contributed to the damaging effects of flavonoids on MCF-7 cells. Total flavonoids also down-regulated the expression of NF-κB, which in turn could down-regulate the expression of bcl-2. By contrast, the expression levels of p53 and fasL were not markedly changed. These findings suggest that induction of the mitochondrial pathway is crucial for flavonoid-induced apoptosis of MCF-7 cells (Fig. 6).


Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
The expression of protein in MCF-7 cells treated with T. chinensis flavonoids detected using western blot analysis. 1, control group; 2, 0.0991 mg/ml; 3, 0.1982 mg/ml; 4, 0.3964 mg/ml; 5,0.7928 mg/ml; 6, 1.5856 mg/ml.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998182&req=5

f6-ol-0-0-4898: The expression of protein in MCF-7 cells treated with T. chinensis flavonoids detected using western blot analysis. 1, control group; 2, 0.0991 mg/ml; 3, 0.1982 mg/ml; 4, 0.3964 mg/ml; 5,0.7928 mg/ml; 6, 1.5856 mg/ml.
Mentions: In order to study the mechanism of flavonoid-induced apoptosis in MCF-7 cells, the expression of caspases, fasL, p53 and bcl-2 were determined. After 24 h of exposure to flavonoids, the expression of cleaved caspase-3 and caspase-9 increased in a dose-dependent manner. Bcl-2 is a crucial determinant of cell apoptosis, and a decrease in its expression was observed in the flavonoid-treated cells. This observation suggests that an intrinsic apoptotic signaling contributed to the damaging effects of flavonoids on MCF-7 cells. Total flavonoids also down-regulated the expression of NF-κB, which in turn could down-regulate the expression of bcl-2. By contrast, the expression levels of p53 and fasL were not markedly changed. These findings suggest that induction of the mitochondrial pathway is crucial for flavonoid-induced apoptosis of MCF-7 cells (Fig. 6).

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.